Figure 1.
Colonization kinetics of Salmonella enterica serovar Typhimurium purA ssaGH in systemically infected BALB/c mice.
Data are shown for spleen (A) and liver (B) of individual untreated mice (open circles), and mice that were treated from day two post infection with enrofloxacin (filled circles). Statistical significance of clearance at day 6 compared to day 4 were determined by t-test of log-transformed data (**, P<0.01; n.s., not significant).
Figure 2.
Competitive indices (CI) of various Salmonella mutants vs. the parental Salmonella purA ssaGH strain in infected spleen (open circles) and liver (filled circles).
Data are shown for individual mice at day seven post infection. A competitive index of 1 indicates equal colonization capabilities of mutant and parental strains. Statistical significance was determined by t-test of log-transformed data. Spleen colonization of mutants ppk, recA, trxA, ubiC, and fadD fadK was significantly lower compared to the parental strain (P<0.05). Liver colonization of mutants ppk, recA, and fadD fadK was significantly lower compared to the parental strain (P<0.05).
Figure 3.
Colonization kinetics of four compromised mutants in spleen (open circles) and liver (filled circles).
Small residual colonization levels after seven days of infection suggested that all shown genes contributed to Salmonella survival but were not absolutely essential. Statistical significance of clearance at day 7 compared to day 1 in spleen was determined by t-test of log-transformed data (***, P<0.001).
Figure 4.
Clearance of Salmonella purA ssaGH fabB from infected mice.
A) Colonization kinetics in spleen (open circles) and liver (filled circles). Similar results were obtained in three independent experiments. Statistical significance of clearance at day 7 compared to day 1 were determined by t-test of log-transformed data (***, P<0.001; **, P<0.01; *, P<0.05; n.s., not significant). B) Heterogeneity of colony size on agar plates. Similar data were obtained for two independent in vitro cultures and five independent ex vivo cultures.