Figure 1.
L. monocytogenes infection impairs chill coma recovery.
Representative chill coma recovery graphs for Days 1–5 post- L. monocytogenes infection for (A) Oregon-R and (B) w1118, The y-axis indicates the percentage of flies that have recovered from chill coma such that they have stood by the time indicated in minutes on the x-axis.
Figure 2.
S. pneumoniae infection impairs chill coma recovery.
Representative chill coma recovery graphs for S. pneumoniae infected (A) Oregon-R and (B) w1118. The y-axis indicates the percentage of flies that have recovered from chill coma such that they have stood by the time indicated in minutes on the x-axis. There is a significant increase in chill recovery time for infected flies compared to their PBS injected controls.
Figure 3.
Chill coma recovery time does not correlate with pathogen load for L. monocytogenes-infected Oregon-R.
Bacterial load was calculated for the five quickest and slowest flies to recover each day throughout the course of infection. There is no statistical correlation between the two (r2 = 0.0243).
Figure 4.
Oregon-R, but not w1118, flies show L. monocytogenes infection-related deficits in negative geotaxis.
Mean and standard error of distance climbed by 4 s post-startle for a representative trial of L. monocytogenes infected (A) Oregon-R and (B) w1118 over the course of infection. Only Oregon-R flies show a significant decline in climbing with infection (*p<0.05, **p<0.01, ***p<0.001).