Figure 1.
Mild decrease voluntary-wheel performance in Myo-PGC-1α animals.
A.) 10 to 12 week old Myo-PGC-1αKO mice and control littermates were individually housed in voluntary running wheel cages with electronic monitoring system for 2 weeks. Tracing of wheel activity, in revolutions per minute is shown. B.) Average number of revolutions per minute C.)Total distance ran in kilometers (km). Error bars indicate s.e.m.; n >6 per group in all panels. * - P<0.05 compared to control.
Figure 2.
Induction of OXPHOS genes in Myo-PGC-1α animals.
A.) After overnight bout of voluntary running wheel, RNA was prepared from quadriceps muscles of the Myo-PGC-1αKO (red bar) and littermate controls (white bar), and the expression of the indicated genes measured by quantitative RT-PCR. B.) After 2 week bout of voluntary running wheel, RNA was prepared from quadriceps muscles of the Myo-PGC-1αKO (red bar) and littermate controls (white bar), and the expression of the indicated genes measured by quantitative RT-PCR. Error bars indicate s.e.m.; n >3 per group in all panels. * - P<0.05 compared to control; # - P<0.05 compared to sedentary.
Figure 3.
Exercise induced mitochondrial biogenesis in Myo-PGC-1α animals.
A.) Schematic of mid-portion of muscle recruited in response to exercise. Shading represents oxidative portion of muscle. Black square represents region of interest (ROI) used for studies B.) Transmission electron micrographs (TEM) of transverse sections of recruited ROI of quadriceps before and after 2-week voluntary wheel run C.) Quantification of mitochondrial density from TEMsof the Myo-PGC-1αKO (red bar) and littermate controls (white bar).n>4 fields from 4 animals per group. Error bars indicate s.e.m.; n >3 per group in all panels. * - P<0.05 compared to control.
Figure 4.
Intact Electron Complex Activity in Myo-PGC-1α in response to exercise.
A.) Enzymatic traces of complex activities B.) Rotenone sensitive NADH dehydrogenase activity (Complex I). C.) Succinate-cytochrome creductase activity (Complex II+III). D.) Glycerol-3-phosphate dehydrogenase + Complex III, rate dependent on glycerol-3-phosphate dehydrogenase (Complex III). E.) Cytochrome oxidase activity (Complex IV).F.) ATPase activity (Complex V). Myo-PGC-1αKO (red bar) and littermate controls (white bar). Error bars indicate s.e.m.; n >3 per group in all panels. * - P<0.05 compared to control; # - P<0.05 compared to sedentary.