Figure 1.
Membrane fatty acid composition of PHM1-41 cells treated with 10 µM, 30 µM, 100 µM of OA, AA, DHA.
Membrane (A) 18∶1 OA, (B) 20∶4 AA, (C) 22∶6 DHA are shown as a percent of total membrane fatty acids. Values are mean percent ± SEM (n = 3 experiments). Bars not sharing common letters are significantly different between treatment groups, p<0.05.
Figure 2.
Representative [Ca2+]i traces recorded from PHM1-41 cells.
Cells were treated with (A) 50 µM BSA, (B) 100 µM OA, (C) 100 µM AA, (D) 100 µM DHA. Arrows indicate application of 25 nM oxytocin (solid) and 100 nM thapsigargin (dashed). Values are mean of 23–26 cells.
Figure 3.
The change in [Ca2+]i of PHM1-41 cells in response to 25 nM oxytocin.
The response of all cells are shown in (A) and cells unresponsive to oxytocin stimulation are excluded from the analysis in (B). Data are expressed as the mean peak increase in [Ca2+]i per dish. Values are mean ± SEM (n = 7 experiments at 10 µM and 30 µM; n = 12 experiments at 100 µM). Bars not sharing common letters are significantly different between treatment groups, p<0.05.
Figure 4.
Total inositol phosphates (IPs) generated in response to 100 nM oxytocin in PHM1-41 cells.
Data are expressed as a percent increase of total IPs over unstimulated basal. Values are mean ± SEM (n = 4 experiments). Bars not sharing common letters are significantly different between treatment groups, p<0.05.
Figure 5.
Internalized [3H]-oxytocin in PHM1-41 cells.
Data are expressed as a percent of total cell-associated ligand (membrane bound+internalized). Values are mean ± SEM (n = 3 experiments). Points not sharing common letters are significantly different between treatment groups, p<0.05.
Table 1.
Maximal oxytocin binding capacity (Bmax) and binding affinity (Kd) in crude PHM1-41 cell membrane preparations.