Table 1.
General information on samples and measurement results.
Figure 1.
Comparing the individual miRNA concentrations between serum and plasma.
The average concentrations (40-Ct values) of detected miRNAs in all serum and plasma samples within each platform were plotted – each point represents the average from all individuals of a specific miRNA. The Y-axis represents the average miRNA concentrations in serum and X-axis is the average concentration of corresponding plasma. The results from Taqman are showed in panel A and Exiqon are showed in panel B.
Figure 2.
Examples of miRNA concentration differences between serum and plasma using individual Taqman QPCR primers.
The sample IDs were listed on the X-axis and the miRNA concentrations were displayed on the Y-axis (in 40-Ct value). The miRNA IDs were indicated on top of the graph. Open bars represent plasma samples and the solid bars represent the corresponding serum samples. The values of standard derivation were obtained from three independent measurements. The original measurement results from Taqman card showed higher 40-Ct values since a pre-amplification step was employed. Two-way ANOVA was used to determine the statistical significance of the miRNA concentration differences between serum and plasma (p-values are shown in the figure).
Table 2.
The list of miRNAs with the most and least concentration variations in serum and plasma samples and measurement platforms.
Figure 3.
Examples of miRNA concentration differences between serum and plasma using individual Exiqon QPCR primers.
The sample IDs were listed on the X-axis and the miRNA concentrations were displayed on the Y-axis (in 40-Ct value). The miRNA IDs were indicated on top of the graph. Open bars represent plasma samples and the solid bars represent the corresponding serum samples. The values of standard derivation were obtained from three independent measurements. Two-way ANOVA was used to determine the statistical significance of the miRNA concentration differences between serum and plasma (p-values are shown in the figure).
Figure 4.
Certain miRNAs showing different measurement efficiency between measurement platforms.
The sample IDs and type of samples were listed on the X-axis. The miRNA concentrations were displayed on the Y-axis (in 40-Ct value). Gray bars represent Exiqon measurement results while solid bars represent the Taqman measurements from the same sample. The values of standard derivation were obtained from three independent measurements.
Table 3.
The list of miRNAs with the most and least concentration variations in different blood cell samples and measurement platforms.