Figure 1.
Flower samples for the Ht-SuperSAGE analysis.
The papaya flowers used for the RNA extraction and Ht-SuperSAGE analysis (P1: male 7-mm flower; P2: male 20-mm flower; P3: female 7-mm flower; P4: female 20-mm flower; P5: hermaphrodite 7-mm flower; and P6: 20-mm hermaphrodite flower).
Table 1.
Summary of the Ht-SuperSAGE analysis.
Figure 2.
Functional categories of the genes annotated from the SC-tags.
Genes corresponding to 456 SC-tags were categorized according to their function, based on their annotation as described in the Methods section. “No match” indicates the tags that had no significant similarities to any non-redundant protein sequences in the databases. “Unknown function” indicates those genes for which the functions of their encoded proteins could not be determined. The SC-tags with different annotations were grouped into the corresponding categories.
Figure 3.
Confirmation of sex-dependent gene expression for Cp2671, which encodes a putative MADS-box protein.
Figure 4.
Structure and expression analysis of the genes corresponding to the Cp3177 tag, which encodes a putative monodehydroascorbate reductase (MDAR).
A) RT-PCR analysis of the MDAR gene corresponding to the Cp3177 tag. P1 to P6 correspond to the flower samples indicated in Figure 1. An actin gene was used as a constitutively expressed control gene [32]. B) A list of the polymorphic tags and their counts for the MDAR genes in the Ht-SuperSAGE data. The polymorphic sequences among the tags are underlined. C) The regions flanking the Cp3177 tag on the BAC clones 46O19 (X chromosome) and 90D06 (Yh chromosome). The arrows indicate the locations of the PCR primers used for amplification. The black regions represent the predicted exons. An insertion of the retroelement sequence was observed in the 90D06 sequence (Yh chromosome). D) Genomic PCR amplification of the MDAR gene in each sex type. The smaller band was equally amplified in all of the sex types. The larger bands, indicating the insertion of retroelements, were only observed in males and hermaphrodites.