Table 1.
Sources and 18S rRNA sequence accessions of microalgae strains used in this study.
Figure 1.
Epifluorescent (A, C, E, G, I, K, M, O, Q, S, U) and differential interference contrast (B, D, F, H, J, L, N, P, R, T, V) images of eleven microalgae used in this study.
Chlorella sp. BR2 (A, B), Nannochloropsis sp. BR2 (C, D), Chaetoceros muelleri (E, F), Chaetoceros calcitrans (G, H), Pavlova lutheri (I, J), Pavlova salina (K, L), Isochrysis sp. (M, N), Dunaliella salina (O, P), Tetraselmis chui (Q, R), Tetraselmis sp. M8 (S, T) and Tetraselmis suecica (U, V). All images were taken at 100x magnification. Bars represent 20 µm.
Table 2.
Growth rate analysis of eleven microalgae strains during growth phase (7 days) of batch culture.
Figure 2.
Maximum likelihood phylogenetic tree of 18S rRNA gene sequences from microalgae used in this study.
Selected sequences from the NCBI database were also included (see Methods for selection criteria). Microalgae analyzed in this study are shown in bold. Numbers represent the results of 100 bootstrap replicates.
Figure 3.
Growth curves of different microalgae in this study.
T. chui, T. suecica, Tetraselmis sp. M8, D. salina, P. salina and Chlorella sp. BR2. Shown are average cell densities ± SD from three biological replicates.
Figure 4.
Growth curves of different microalgae in this study.
C. calcitrans, C. muelleri, I. galbana, Nannochloropsis sp. BR2, Chlorella sp. BR2, P. lutheri & Tetraselmis sp. M8 (Outdoors). Shown are average cell densities ± SD from two biological replicates (3 replicates for Nannochloropsis sp. BR2 & 1 for Tetraselmis sp. M8 (Outdoors)).
Figure 5.
FAME levels of microalgae strains grown in batch culture
(7 days growth + 2 days starvation by replacement of medium with seawater). Values shown are the averages of three biological replicates ± SD (except Tetraselmis sp.1). Different superscripts indicate significant difference at 95% level (ANOVA, Bonferroni's test; P<0.05). 1Mid-scale outdoors culture.
Table 3.
Fatty acid composition in percentage of total FAME of different subtropical Australian microalgae strains after batch culture (7 days growth +2 days starvation).
Table 4.
Comparison of FAME productivity (μg mL−1 day−1) of present study microalgae with lipid productivity of microalgae species from other references.