Figure 1.
Filovirus species-specificity of IgG antibodies detected in orangutan sera.
Serum samples diluted 1∶100 were tested for IgG antibodies reacting with soluble GP antigens in ELISA (A) and with GP, NP, and VP40 in Western blotting (B). Representative data for ZEBOV (#307), SEBOV (#116), CIEBOV (#340), BEBOV (#304), REBOV (#182), MARV (#106), and multiple (#214) GP-positive samples are shown. Asterisks indicate significantly higher OD values determined by the Smirnov-Grubbs rejection test (P < 0.01). VLPs consisting of GP, NP, and VP40 were used for Western blotting, and rabbit and mouse antisera or mouse monoclonal antibodies ZGP43/3.7 and AGP127-8 was used as positive controls as described in Materials and Methods. 1, ZEBOV; 2, SEBOV; 3, CIEBOV; 4, BEBOV; 5, REBOV; 6, MARV; 7, negative control.
Table 1.
Serum IgG antibody titers of the positive sera.
Table 2.
Filovirus species specificity of the serum IgG antibodies detected in orangutans in East and Central Kalimantan.
Table 3.
Seroprevalence of filoviruses in orangutans in East and Central Kalimantan.