Figure 1.
Normalized PCA modes (A) of a single cell (B) of a paraformaldehyde fixed cell (C) of a group of n = 198 cells. The spatial distribution of the normalized mode amplitude is color coded. (D) Phase image of erythrocyte with typical ROI for PCA analysis (E) Eigenvalues of PCA modes of A,B,C (Log Scale). Standard deviations of the values are indicated by error bars.
Figure 2.
Straigth line: Equilibrium shape that arises from bending energy minimization (see Text S1) and which was used in the calculations to derive eigenmodes of the bending deformation energies depicted in Figure 3B,C. Dashed line: Shape of deformed cell.
Figure 3.
Experimental and Theoretical Comparison.
(A) Degeneracy free representation of PCA modes. The separable modes 7 (degeneracy of 2) and 9 in this representation are obtained from an appropriate linear combination of the degenerate modes 12,13 and 14 of Figure 1C. Both representations are equivalent, because they span the same subspace. (B) Eigenmodes that minimize Laplace deformation energy shown in the ROI. Eigenmodes are plotted as functions (C) Eigenmodes that minimize BCH Deformation Energy shown only in the ROI (D) Comparison of theoretical and measured fluctuation amplitudes.
Figure 4.
(A) Fluctuation amplitudes of the first six PCA modes in a population of n = 198 (no ATP depletion), 43 (1 h ATP depletion), 47 (2 h ATP depletion), 36 (4 h ATP depletion) cells. (B) Assessment of intracellular ATP level at different instants of incubation time in depletion medium through the measurement of chemiluminescence intensity of luciferin luciferase assay in RBC cell solution.