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Figure 1.

Flow diagram of the study.

IgA nephropathy was diagnosed in 436 patients between January 2000 and December 2008. Excluding 93 patients, a total of 343 patients were enrolled. eGFR, estimated glomerular filtration rate; GN, glomerulonephritis; SLE, systemic lupus erythematosus.

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Figure 2.

Representative pictures of immunofluorescence staining of mesangial C3 1+ to 3+.

Immunofluorescence intensity was quantified by ImageJ software.

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Figure 3.

A scattered plot of each level of serum C3 between patients with C3 levels <90 mg/dl and patients with C3 levels ≥90 mg/dl.

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Table 1.

Demographic, clinical and biochemical characteristics.

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Figure 4.

The histopathologic grades such as (A) mesangial hypercellularity, (B) segmental glomerulosclerosis, (C) endocapillary hypercellularity, and (D) tubular atrophy/interstitial fibrosis according to mesangial C3 deposition.

Mesangial hypercellularity (C3 deposition 0, 9.4%; 1+, 29.7%; 2+∼3+, 49.3%; P<0.001) and high-grade tubular atrophy/interstitial fibrosis (C3 deposition 0, 7.5%; 1+, 10.5%; 2+∼3+, 14.1%; P<0.001) were more prominent as the mesangial area of C3 deposition increased.

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Figure 5.

Comparison of serum C3 levels according to mesangial C3 deposition.

Serum C3 levels decreased significantly from 0 to 2+∼3+ mesangial C3 deposition (0, 111.7±18.0; 1+, 104.3±17.1; 2+∼3+, 98.6±14.2 mg/dl; P<0.001).

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Table 2.

Comparison of histopathologic features from renal biopsies between patients with and without decreased C3 levels.

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Figure 6.

Kaplan-Meier analyses of cumulative renal survival of patients with IgA nephropathy based on (A) serum C3 level and (B) mesangial C3 deposition.

(A) A 10-year renal survival rate was significantly lower in patients with C3 levels <90 mg/dl than those with C3 levels ≥90 mg/dl (P = 0.006). (B) A 10-year survival in patients with 2+ and 3+ mesangial deposition of C3 was lower than in those without C3 deposition (P = 0.04).

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Figure 7.

ROC curve analysis for renal outcome of the doubling of the baseline serum creatinine.

Serum C3 levels had a significant predictive value for renal outcome (AUC = 0.642, P = 0.011), although the predictive value of serum C3 was lower than UPCR (AUC = 0.819, P<0.001) or eGFR (AUC = 0.781, P<0.001).

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Figure 8.

Kaplan-Meier analyses of cumulative renal survival of patients with IgA nephropathy according to histopathologic features including (A) mesangial hypercellularity, (B) segmental glomerulosclerosis, (C) endocapillary hypercellularity, and (D) tubular atrophy/interstitial fibrosis.

Patients with mesangial hypercellularity, segmental glomerulosclerosis, endocapillary hypercellularity, and tubular atrophy/interstitial fibrosis had significantly lower renal survival than those without such findings (P<0.05).

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Table 3.

Incidence of patients with doubling of serum creatinine and ESRD according to decreased C3 levels and mesangial C3 deposition.

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Table 4.

Cox regression models for renal outcome of the doubling of the baseline serum creatinine.

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Figure 9.

The decline rate of eGFR. Error bars indicate standard error.

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