Figure 1.
Flow-chart showing available drug susceptibility testing (DST), sequencing, and multiplex allele-specific PCR (MAS-PCR) results for the 98 archived isolates from Panama.
Figure 2.
Frequency of the five most common INH and RIF resistance mutations among 67 Panamanian MDR-TB isolates.
Table 1.
Pairwise comparisons of the three drug resistance detection techniques (DST, DNA sequencing, and MAS-PCR) in detecting INH and RIF resistance.
Figure 3.
Distinct band patterns indicate drug resistance profile of isolates.
A. Loci where each allele-specific primer binds are indicated, along with the expected product size if the locus is wild-type. The two common mutations that confer resistance to INH and the three common mutations that confer resistance to RIF are boxed separately. B. Band patterns indicate drug resistance profile of isolates. Expected PCR products have been color-coded in the same way as in A. Lane 1: H37Rv reference strain (wild-type at all 5 loci); Lane 2: mabA-inhA −15C→T and RpoB D516F double mutant; Lane 3: KatG S315T and RpoB H526Y double mutant; Lane 4: KatG S315G and RpoB 531L double mutant; Lane 5: mabA-inhA −15C→T, KatG S315T and RpoB H526D triple mutant; lane 6: mabA-inhA −15C→T, KatG S315T and RpoB S531L triple mutant; Lane 7: Molecular ladder.
Table 2.
Primers for MAS-PCR to detect INH and RIF resistance.