Figure 1.
Laurdan generalized polarization:
Laurdan generalized polarization in unilamellar vesicles of (-□-) DPPC; (-○-) DOPC and (-Δ-) DOPC/DPPC as a function of cholesterol content at (A) 20°C; (B) 37°C; (C) 45°C and (D) 50°C. Each point represents the average and standard error of triplicate data.
Figure 2.
DPH fluorescence lifetime distribution center in LUVs of (-□-) DPPC; (-○-) DOPC and (-Δ-) DOPC/DPPC as a function of cholesterol content at (A) 20°C; (B) 37°C; (C) 45°C and (D) 50°C. Each point represents the average and standard error of triplicate data.
Figure 3.
Laurdan fluorescence lifetime:
Laurdan fluorescence lifetime distribution center in unilamellar vesicles of (-□-) DPPC; (-○-) DOPC and (-Δ-) DOPC/DPPC as a function of cholesterol content at (A) 20°C; (B) 37°C; (C) 45°C and (D) 50°C. Each point represents the average and standard error of triplicate data.
Table 1.
Anisotropy decay parameters in unilamellar vesicles.
Figure 4.
Laurdan generalized polarization images of the DOPC/DPPC (1∶1 mol%) mixtures with different cholesterol content. The immiscibility transition temperature is indicated for each mixture. Different GP values are represented by an artificial color bar.
Figure 5.
Thermotropic behavior of average Laurdan GP histograms values in the DOPC/DPPC (1∶1 mol%) mixture with (A) 0, (B) 15 mol%, (C) 22.2 mol% and (D) 33.3 mol% cholesterol.
Figure 6.
Temperature at phase separation:
Immiscibility transition temperatures (-▪-) and Laurdan GP value (-Δ-) at which the immiscibility occurs as a function of bilayer cholesterol content.
Figure 7.
Laurdan fluorescence GP in GUVs of DOPC/DPPC (1∶1 mol%) as a function of cholesterol content, obtained from two-photon microscopy GP images at different temperatures. The symbols correspond to (-▪-)the high GP value at 25°C, (-□-) the low GP value at 25°C, (-○-) the GP value at 37°C, (-Δ-) the GP value at 41°C, (-◊-) the GP value at 50°C and (-•-) the GP value in LUVs of DOPC/DPPC (1∶1 mol%) as a function of cholesterol content.