Table 1.
List of M. tuberculosis isolates used in this study.
Table 2.
MIC of gyrA transductants/mutants.
Table 3.
MIC of gyrB transductants/mutants.
Figure 1.
Amino acid substitutions in M. tuberculosis gyrB.
A, numbering system according to http://genolist.pasteur.fr/TubercuList/annotation, B, numbering system according to [45] C, numbering system according to http://tuberculist.epfl.ch/index.html annotation. Highlighted in bold are the mutations we analyzed in this study.
Figure 2.
A structural model of M. tuberculosis gyrase inhibition.
A. A model of M. tuberculosis gyrase in complex with DNA and levofloxacin. The model was built based on the crystal structure of the complex of Streptococcus pneumoniae (PDB ID: 3K9F) as described in Materials and Methods. The GyrA subunit is shown in yellow, GyrB is in green, DNA is in orange, the levofloxacin molecule is shown as pink sticks. B. A zoomed-in view of the quinolone binding site. Residues that directly interact with the quinolone and whose mutations cause resistance are shown by blue sticks. The two residues whose double, but not single, mutations cause fluoroquinolone resistance are shown by red sticks.