Table 1.
Clinical characteristics of the patients included in the cohort.
Figure 1.
BT3.2 expression in ovarian cancer cells.
A. Western-blot analysis of total protein extracts from TOV112D cell line infected with PLenti (vector control), BT3.1, BT3.2 or BT3.3 viral constructs. Extracts were loaded in triplicate on 10% SDS/PAGE gel and membranes were hybridized with anti-CD277 (eBioscience), anti-BT3.3 (Atlas Antibodies) and anti-BT3.2 (SDIX Inc.) as indicated at the bottom of the Figure. B. Immunohistochemistry analysis of paraffin-embedded cell pellets from TOV112D cell line infected with either PLenti (vector control), BT3.1, BT3.2 or the BT3.3 viral constructs. Only cells transfected with the BT3.2 construct stained positively with the anti-BT3.2 (SDIX Inc.). C. Immunohistochemistry of xenograft tumors from TOV112D transfected with either empty vector or BT3.2 construct. Only cells infected with the BT3.2 construct stained positively with the anti-BT3.2 (SDIX Inc.). D. Representative staining for immunohistochemistry of BT3.2 on a high-grade serous EOC TMA. From left to right: negative, low, moderate and high intensity.
Table 2.
Pearson correlation test (two-tailed) between BT3.2 expression in EOC tissues and clinical parameters of patients.
Figure 2.
Kaplan Meier analysis of BT3.2 in high-grade serous EOC.
Kaplan-Meier curves of overall survival (A) and disease-free survival (B) in 194 and 171 patients, respectively. Significance (p) is indicated by log rank.
Table 3.
Univariate and multivariate Cox regression analysis showing the statistical association between BT3.2 expression and outcome of EOC patients.
Figure 3.
Representative staining for immunohistochemistry of tumor infiltrating lymphocytes.
High and low density of CD3+ (T cells), CD4+ (T cells), CD8+ (T cells) and CD20+ (B cells) shown in left and middle panels. A magnification in the high infiltration area is shown on the right panel for each staining.
Table 4.
Pearson correlation test (two-tailed) between BT3.2 expression and markers of intra-epithelial immune infiltrate in EOC tissues.
Figure 4.
Representative staining for immunohistochemistry of infiltrating macrophages.
High and low density of CD68+ (tumor-associated macrophages, TAM) (A) and CD206+ (M2 subtype of TAM) cells (B). Magnification in the high infiltration area is shown on the right panel for each staining (top). C and D. Kaplan-Meier analysis of the ratio of infiltrating intraepithelial CD206+/CD68+ cells representing the relative density of CD206+ M2 TAM over the total density of CD68+ intraepithelial infiltrating macrophages. Kaplan-Meier curves of overall survival (C) and disease-free survival (D) in 180 and 174 patients, respectively. Significance (p) is indicated by log rank.
Table 5.
Pearson correlation test (two-tailed) between intra-epithelial immune infiltrate and clinical parameters in EOC patients.