Table 1.
The content of CYP3A4 and CYP2B6 co-expressed with wild-type or six POR mutants in sf9 microsomal fractions were determined on the basis of reduced CO-difference spectrum.
Table 2.
The reductive activity of wild-type and variant POR expressed alone and the activity of POR co-expressed with CYP3A4 or CYP2B6 in sf9 was estimated by NADPH-dependent Cytochrome c reduction.
Figure 1.
Determination of enzymatic activities of CYP3A4-PORs.
Kinetics for the formation of hydroxytestosterone was determined by incubation of testosterone with CYP3A4–PORs, as described in method. Data are depicted as mean±S.D. (n = 3). The insert graphs show the Lineweaver–Burk plot of the data.
Table 3.
Km and Vmax values for CYP3A4 and CYP2B6 enzymes with different mutants of POR were determined by their specific substrates testosterone and bupropion.
Figure 2.
Determination of enzymatic activities of CYP2B6-PORs.
Kinetics for the formation of hydroxybupropion was determined by incubation of bupropion with CYP2B6-PORs, as described in Method. Data are depicted as mean±S.D. (n = 3). The insert graphs show the Lineweaver–Burk plot of the data.