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Figure 1.

Total root length of maize plants during the whole growth period in response to N fertilization in three consecutive years.

In 2007, the whole root system was excavated with a soil volume of 28 cm×35 cm and a depth of 40 cm. In 2008 and 2009, root systems were excavated within a soil volume of 28 cm×35 cm and a depth of 50 cm (2008) or 60 cm (2009) with 10 cm increments. The bars represent the standard error of the mean, n = 4. TDAT means N top dressing after tasseling. The total amount of N applied in TDAT treatment was 230 and 250 kg ha−1 in 2007 and 2008, respectively. TDBT means N top dressing before tasseling. The total amount of N applied in TDBT treatment was 395, 250 and 250 kg ha−1 in 2007, 2008 and 2009, respectively, and is the same in the following figures.

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Figure 2.

Length of embryonic roots and different whorls (1st to 7th orders) of nodal roots of maize plants in response to N fertilization in 2007.

Arrows indicate the time of tasseling. Whole root systems were excavated with a soil volume of 28 cm×35 cm and a depth of 40 cm, and then separated into embryonic roots and different whorls of nodal roots. The bars represent the standard error of the mean, n = 4.

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Figure 3.

Total root length of maize plants in each soil layer at different growth stages in response to N fertilization in 2008.

Root systems were excavated within a soil volume of 28 cm×35 cm and a depth of 50 cm with 10 cm increments. The bars represent the standard error of the mean, n = 4.

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Figure 3 Expand

Figure 4.

Total root length of maize plants in each soil layer at different growth stages in response to N fertilization in 2009.

Roots were excavated within a soil volume of 28 cm×35 cm and a depth of 60 cm with 10 cm increments. The bars represent the standard error of the mean, n = 4.

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Figure 4 Expand

Table 1.

Shoot dry matter accumulation (t/ha) in different growth periods, final shoot dry weight (DW) and grain yield (t/ha) of maize plants supplied with different N rates in three years.

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Table 2.

Shoot N accumulation (kg/ha) in different growth periods and the final shoot N content of maize plants supplied with different N rates in three years.

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Figure 5.

Soil mineral nitrogen (Nmin; NH4+-N+NO3-N) in the 0–90 cm soil profile in response to N fertilization in 2007.

The soil samples were obtained using the soil auger method at the same time in each plant harvest. The bars represent the standard error of the mean, n = 4.

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Figure 6.

Temporal and spatial distribution of soil mineral nitrogen (Nmin; NH4+-N+NO3-N) in the 0–50 cm soil profile in response to N fertilization in 2008.

The soil samples were obtained by excavating soil layers within a soil volume of 28 cm×35 cm and a total depth of 50 cm with 10 cm increments at each time after shoot harvest. The bars represent the standard error of the mean, n = 4.

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Figure 7.

Temporal and spatial distribution of soil mineral nitrogen (Nmin; NH4+-N+NO3-N) in the 0–60 cm soil profile in response to N fertilization in 2009.

The soil samples were obtained by excavating soil layers within a soil volume of 28 cm×35 cm and a total depth of 60 cm with 10 cm increments at each time after shoot harvest. The bars represent the standard error of the mean, n = 4.

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Figure 7 Expand

Table 3.

Ratio of DW and N uptake presilking and total accumulation in maize plants with different N treatments in 2007, 2008 and 2009.

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Table 3 Expand