Figure 1.
The bioreduction of TMSB to (S)-TMSBOL with immobilized Candida parapsilosis CCTCC M203011 cells in various IL-containing co-solvent systems.
Table 1.
Effect of various hydrophilic ILs on the bioreduction of TMSB with immobilized Candida parapsilosis cells.
Figure 2.
Cell viability of Candida parapsilosis.
Conditions: Cells were exposed to co-solvent systems comprising 10% (v/v) of various ILs and TEA-HCl buffer (100 mmol/L, pH 5.0), with and without substrate (3.0 mmol/L TMSB).
Figure 3.
Effect of C2OHMIM·NO3 content on the bioreduction of TMSB.
Symbols: (△) the initial reaction rate; (□) the maximum product yield; (○) the product e.e. All products have the (S) configuration. Reaction conditions: 3.0 mmol/L TMSB, 4.0 mL TEA-HCl buffer (100 mmol/L, pH 5.0) containing different amount of C2OHMIM·NO3, 65.3 mmol/L 2-propanol, and 0.15 g/mL immobilized C. parapsilosis cells, 30°C, 180 r/min.
Table 2.
Effect of substrate concentration on the bioreduction of TMSB with immobilized Candida parapsilosis cells.
Figure 4.
Effect of buffer pH on the bioreduction of TMSB.
Symbols: (△) the initial reaction rate; (□) the maximum product yield; (○) the product e.e. All products have the (S) configuration. Reaction conditions: 3.0 mmol/L TMSB, 4.0 mL TEA-HCl buffer (100 mmol/L, various pH) containing 10% (v/v) C2OHMIM·NO3, 65.3 mmol/L 2-propanol, and 0.15 g/mL immobilized C. parapsilosis cells, 30°C, 180 r/min.
Table 3.
Effect of co-substrate concentration on the bioreduction of TMSB with immobilized Candida parapsilosis cells.
Figure 5.
Effect of reaction temperature on the bioreduction of TMSB.
Symbols: (△) the initial reaction rate; (□) the maximum product yield; (○) the product e.e. All products have the (S) configuration. Reaction conditions: 3.0 mmol/L TMSB, 4.0 mL TEA-HCl buffer (100 mmol/L, pH 5.0) containing 10% (v/v) C2OHMIM·NO3, 98.1 mmol/L 2-propanol, and 0.15 g/mL immobilized C. parapsilosis cells, various reaction temperatures, 180 r/min.
Figure 6.
Operational stability of immobilized Candida parapsilosis cells.
Reaction conditions with C2OHMIM·NO3-buffer system: 3.0 mmol/L TMSB, 4.0 mL TEA-HCl buffer (100 mmol/L, pH 5.0) containing 10% (v/v) C2OHMIM·NO3, 98.1 mmol/L 2-propanol, and 0.15 g/mL immobilized C. parapsilosis cells, 30°C, 180 r/min, 1 h per batch; Reaction conditions with aqueous buffer system: 3 mmol/L TMSB, 4.0 mL of TEA-HCl buffer (100 mmol/L, pH 5.0), 65.3 mmol/L 2-propanol, 0.15 g/mL immobilized C. parapsilosis cells, 30°C, 180 r/min, 1 h per batch. The relative activity of the immobilized cells in the first batch was defined as 100%.