Figure 1.
Infected SupT1/PBMC coculture and uninfected control.
Infection was done with a virus input of 60 ng CA-p24 wild type HIV-1 LAI virus. In order to distinguish natural cell death from virus-mediated cell death (especially important for primary CD4+ T cells due to lower vitality and proliferation than SupT1 cells), the virus-mediated cell killing was determined by comparing the number of live cells in the infected coculture with the number of live cells in the uninfected control coculture. The results shown are from a representative experiment. The experiment was repeated ten times, and similar results were obtained (see Fig. 4 and Fig. 5 for details).
Figure 2.
Infected PBMC culture and uninfected control.
Infection was done with a virus input of 60 ng CA-p24 wild type HIV-1 LAI virus. In order to distinguish natural cell death from virus-mediated cell death, the virus-mediated cell killing was determined by comparing the number of live cells in the infected culture with the number of live cells in the uninfected control culture. The results shown are from a representative experiment. The experiment was repeated ten times, and similar results were obtained (see Fig. 4 and Fig. 5 for details).
Figure 3.
Infected SupT1 culture and uninfected control.
Infection was done with a virus input of 60 ng CA-p24 wild type HIV-1 LAI virus. The virus-mediated cell killing was determined by comparing the number of live cells in the infected culture with the number of live cells in the uninfected control culture. The results shown are from a representative experiment. The experiment was repeated ten times, and similar results were obtained (see Fig. 4 and Fig. 5 for details).
Figure 4.
Data regarding repeatability of the experiments.
All experiments in this study were repeated ten times; in each experiment, successive repetitions yielded consistent results. The collected data were summarized using means and standard deviations. This figure shows the data regarding the infected cultures. In the graphs, the lines show the mean values, and the error bars represent the standard deviations.
Figure 5.
Data regarding repeatability of the experiments.
All experiments in this study were repeated ten times; in each experiment, successive repetitions yielded consistent results. The collected data were summarized using means and standard deviations. This figure shows the data regarding the uninfected cultures. In the graphs, the lines show the mean values, and the error bars represent the standard deviations.
Figure 6.
Examination of the difference in primary CD4+ T cell survival between the infected SupT1/PBMC cocultures and the infected PBMC cultures.
In the graph, the lines show the mean values, and the error bars represent the standard deviations. Statistical significance (p) was assessed using the unpaired Student's t test.