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Table 1.

The anti-P. falciparum activities of BAQ and MAQ determined in parallel with chloroquine, by the ELISA anti-HRPII assay or by 3H hypoxanthine incorporation.

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Table 1 Expand

Table 2.

Cytotoxicity of BAQ and MAQ against a human hepatoma cell line (HEPG2) and a monkey kidney cell line (BGM) determined by the MTT assay.

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Table 3.

Antimalarial activity of BAQ and MAQ in mice infected with P. berghei after treatment with daily doses of the compounds during three consecutive days.

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Table 3 Expand

Figure 1.

Inhibition of hemozoin formation by the CQ analogs BAQ and MAQ (mean ± SD from triplicates), in two different experiments.

Statistical differences as compared to drug-free controls are indicated in each graph by an asterisk (p<0.05). The p and r values represent the statistical correlation analyses.

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Figure 1 Expand

Figure 2.

Chemical structures of the protonated forms of the CQ analogs BAQ and MAQ.

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Figure 2 Expand

Figure 3.

Compounds docked in dimeric hematin.

(A) Protonated CQ, (B) MAQ-1, (C) MAQ-2, (D) MAQ-3, (E) BAQ-1, (F) BAQ-2.

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Figure 3 Expand

Table 4.

Docking energies of the protonated forms of the CQ analogs BAQ and MAQ.

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Table 4 Expand

Figure 4.

Best conformations of the protonated forms of chloroquine, MAQ and BAQ (in green) in the binding pocket of NADH (in CPK) as generated by the MVD® software.

(A) Protonated chloroquine, (B) MAQ-1, (C) MAQ-2, (D) MAQ-3, (E) BAQ-1, (F) BAQ-2.

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Figure 4 Expand

Table 5.

Docking results of NADH and the protonated forms of chloroquine, BAQ and MAQ in the active site of PfLDH.

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Table 5 Expand