Table 1.
Antimicrobial susceptibility patterns of A. lwoffii SGC-HZ9, transformants and transconjugants.
Figure 1.
Identification of a mobile blaNDM-1 gene.
(A) PFGE analysis of the plasmid preparations. (B) Southern hybridization of the PFGE gel and S1 nuclease PFGE gel with a blaNDM-1 probe. For both panels, lanes 1–3 contain plasmids prepared from A. lwoffii SGC-HZ9, transformant JM109-SGC-HZ9, and transconjugant J53-SGC-HZ9, respectively. M: Lambda Ladder PFG Marker (NEB, UK).
Figure 2.
Schematic map representing blaNDM-1 and its flanking genetic sequences.
Comparison of plasmid regions around the blaNDM-1 gene in A. lwoffii SGC-HZ9 (JN616388), E. coli 271(HQ162469) [12], K. pneumoniae 05-506 (FN396876) [2], and E. coli HK-01 (HQ451074) [13]. The vertical dotted lines indicate the locations at which the sequences diverge. X and E depict XbaI and EcoRI sites, respectively. The −35 promoter region of blaNDM-1 and the overlapping EcoRI site are indicated on top of the panel. The right inverted repeat of ISAba125 is shaded and partly overlaps with the −35 box of blaNDM-1.
Table 2.
MBL gene primers designed in this study.