Figure 1.
Changes in histology during mouse esophageal development and sampling scheme in this study.
(A) Three esophageal samples in each group at each time point were used for analysis. (B–I) H&E staining of paraffin sections of mouse esophagus showed histological changes of esophageal epithelium and mesenchyme in metaplasia phase (E11.5, E15.5, P0 and P7). Panel F, G, H and I (size bar = 20 µm) are magnifications of Panel B, C, D and E (size bar = 100 µm), respectively. Es, esophagus; Tr, trachea; Ep, epithelium; Me, mesenchyme.
Table 1.
Differential expression of knowledge-based gene sets in the mouse esophagus in the metaplasia phase.
Figure 2.
Involvement of the Wnt pathway in the development of mouse esophageal epithelium.
(A) X-Gal staining of E11.5 and E13.5 esophagi of BAT-GAL mice; (B) X-Gal staining of E13.5 esophagi of TOP-GAL mice. Es, esophagus; Tr, trachea; Ep, epithelium; Me, mesenchyme; Lu, lung.
Figure 3.
Esophageal hyperkeratosis due to Nrf2 superactivation in Keap1−/− esophagus.
P7 esophagi of a wild-type mouse (A), a Nrf2−/− mouse (B), a Keap1−/− mouse (C), and a Nrf2−/−Keap1−/− mouse (D), were stained for H&E. Expression of Nrf2 (E–G), Pparγ/δ (H–J) and pAkt (K–L) were shown in the esophagi of P7 wild-type mouse, P7 Keap1−/− mouse and adult wild-type mouse. Size bar = 50 µm.
Table 2.
Pathway changes in the three phases of mouse esophageal development.