Figure 1.
Flow of participants in the study.
Table 1.
Anthropometric and metabolic responses to overfeeding in the whole cohort and in men and women.
Table 2.
Composition of the diet at baseline and during overfeeding.
Figure 2.
Urinary F2-isoprostane content and skeletal muscle protein carbonyls in response to overfeeding.
Urine F2-isoprostane (A), skeletal muscle protein carbonyls quantification (B) and representative blots (C) and the association between protein carbonyls and peripheral insulin resistance at end of overfeeding (D). Data are expressed as mean±SEM at baseline (white), day 3 (striped) and day 28 (black) of overfeeding. Difference by RM-ANOVA *P<0.05, **P = 0.01, urinary F2-isoprostanes data is based on n = 37 (18 men and 17 women) and skeletal muscle protein carbonyl on n = 18 (8 men and 10 women).
Figure 3.
Skeletal muscle protein expression and enzyme activities in response to overfeeding.
Skeletal muscle complexes of the electron transport chain (a), Mn-superoxide dismutase (SOD), uncoupling protein-3 (UCP3), PPAR-coactivator 1α (PGC1α), and carnitine palmitoyltransferase (CPT1b) proteins and representative samples of the Western blots (b) and skeletal muscle citrate synthase (CS), hydroxyacyl-CoA dehydrogenase (βHAD), hexokinase and phosphofructokinase (PFK) activities (c). Data expressed relative to baseline at day 3 (striped) and day 28 (black) of overfeeding (n = 26; 13 men and 13 women). Difference by one-way t-test *P<0.05, **P<0.01.