Figure 1.
Pesticide induced loss of TH+ neurons and increased α-synuclein immunoreactivity.
A) Immunostaining of SN of PQ, PY, MNPQ and wild type (WT) mice with tyrosine hydroxylase antibody. B) Percent loss of TH+ neurons and fold increase of α-synuclein immunoreactivity compared to wild type control mice.
Figure 2.
Validation of RNA-Seq gene expression fold changes by Taqman real time PCR.
A) Gene expression fold changes in VMB. B) Gene expression fold changes in the STR.
Table 1.
Taqman assays used for quantitative RT-PCR.
Figure 3.
Common genes in the VMB between PQ, PY and MNPQ mouse models of PD.
A) Venn diagram of 500 up-regulated VMB transcripts from PQ, PY and MNPQ models of PD. B) Venn diagram of 500 down-regulated VMB transcripts from PQ, PY and MNPQ models of PD. C) Common up-regulated genes in the VMB of PQ, PY and MNPQ models of PD. D) Common down-regulated genes in the VMB of PQ, PY and MNPQ models of PD.
Figure 4.
Common genes in the STR between PQ, PY and MNPQ mouse models of PD.
A) Venn diagram of 500 up-regulated STR transcripts from PQ, PY and MNPQ models of PD. B) Venn diagram of 500 down-regulated STR transcripts from PQ, PY and MNPQ models of PD. C) Common up-regulated genes in the STR of PQ, PY and MNPQ models of PD. D) Common down-regulated genes in the STR of PQ, PY and MNPQ models of PD.
Figure 5.
Venn diagram of top ten signaling pathways in PQ, PY and MNPQ models of PD.
A) Signaling pathways in VMB. B) Signaling pathways in STR. C) Common overlapping pathways in VMB and STR are underlined. Considering the top ten canonical pathways there are six pathways common in VMB of PQ, PY and MNPQ mouse models. There are three pathways common in STR of PQ, PY and MNPQ mouse models. All the three common canonical pathways in STR overlap with pathways common in the VMB between the three pesticide models.
Table 2.
Overlapping signaling pathways between human PD transcriptome studies and signaling pathways in VMB of PQ, PY and MNPQ mouse models of PD.
Table 3.
Overlapping signaling pathways between human PD transcriptome studies and signaling pathways in STR of PQ, PY and MNPQ mouse models of PD.
Figure 6.
Global gene network in VMB of PQ, PY and MNPQ mouse models of PD.
The genes from the most significant network of each individual pesticide mouse model were overlayed to generate global gene networks in the VMB. Down-regulated genes are highlighted in green, up-regulated genes are highlighted in red. The focus molecules-G protein coupled receptors (GPCRs) members, Kelch-like ECH-associated protein 1 (Keap1) and peroxisome proliferator-activated receptor delta (Pparδ) are down-regulated in VMB.
Figure 7.
Global gene network in STR of PQ, PY and MNPQ mouse models of PD.
The genes from the most significant network of each individual pesticide mouse model were overlayed to generate global gene networks in the STR. Down-regulated genes are highlighted in green, up-regulated genes are highlighted in red. The focus molecules- G protein coupled receptors (GPCRs) members and Pparδ are down-regulated while Atxn1 is up-regulated.