Table 1.
Experimental Subjects.
Figure 1.
Rostral migratory stream in the dog brain.
(A) Schematic of a sagittal view of a canine brain. The red line indicates the location of the RMS in relationship to the anterior horm of the lateral ventricle (LV), caudate nucleus (CN), olfactory bulb (OB), cortex (Ctx) and cerebellum (Cb). (B) Nissl staining showing the orientation and nomenclature of the canine RMS. (C) anti-BrdU immunostaining in brown with hematoxylin counterstain in purple. C1 shows BrdU staining at the boundary of the white matter and caudate nucleus in the descending limb; C2 shows BrdU staining in the olfactory peduncle/rostral limb. (D) anti-Dcx immunostaining. D1 shows morphology of cells in the funnel; D2 shows leading and trailing processes of migrating cells in the descending limb. (E) Immunoreactivity for BrdU (green, in the RMS) and NeuN (red, in the CN) does not overlap in the descending limb. Section is counterstained with DAPI (blue). (F) BrdU immunostaining (brown) in the olfactory peduncle in tissue from dog 5, analyzed at 6 hr after a single 75 mg/kg i.v., indicating that BrdU is taken up by dividing cells all along the RMS. Scale bar in B, C, D: 1 mm. Scale bars in C1, C2, E: 100 microns. Scale bars in D1, D2, F: 50 microns. Please view the figures on a computer monitor for accurate RGB color representation.
Figure 2.
Rostral migratory stream in the cat brain.
(A) Nissl staining demonstrating the RMS orientation and location from the anterior horn of the lateral ventricle (LV) to the olfactory bulb (OB). (B) anti-BrdU immunostaining. (C) anti-Ki67 immunostaining, demonstrating the presence of dividing cells along the entire RMS. (D, E) T2-weighted MRI images of a cat head in the dorsal (D) and transverse (E) planes, showing cerebrospinal fluid in the open olfactory ventricles of an adult (5 year old) cat (arrows). Scale bars in main panels A–D: 500 microns. Scale bars in insets B1–2, C1–3∶100 microns.
Figure 3.
Relationship of white matter and the RMS.
(A) Luxol fast blue staining of a saggital section from dog 4. Inset shows the approximate location of the RMS in red and part of the white matter in blue. (B–D) Confocal maximum projection images of CNPase staining (red) and doublecortin staining (green) in the SVZ (B), funnel (C), and olfactory peduncle (D) in dog 1.
Table 2.
Primary Antibodies.
Figure 4.
Areas of dog brain (A–C) and cat brain (D–E) embedded for sectioning.
(A, D): Lateral view. (C, E): Ventral view. (B): View from the midline. Hemispheres were separated and an ∼40×22×22 mm block (including the olfactory bulb when possible) was isolated from each side (B’–E’). Scale bar in B’ and D’ is 1 cm. Labels are based on the atlas of Singer.