Table 1.
A list of the primers used in this study.
Figure 1.
ELTD1 expression in normal and hypertrophied ventricles.
A, Representative and statistical data of western blotting for protein levels of ELTD1 with glyceraldehyde phosphate dehydrogenase (GAPDH) as loading control (n = 4). *P<0.05 vs. normal donators. Left, representative blots. Right, quantitative results. B, Western blotting for levels of ELTD1 in mice at different tissue indicated (n = 4). C, Real-time PCR for ELTD1 and heart weight/body weight (HW/BW) in WT control mice after AB at time points indicated (n = 6). Data represent as mean±SEM. Up, ratios indicate mRNA levels normalized to GAPDH. #P<0.01 vs. the sham values.
Figure 2.
The effects of ELTD1 on cardiac hypertrophy 28 days post-surgery.
A, Representative gross heart, wall thickness and HE staining in WT and KO mice B, Statistical results of the HW/BW, HW/TL and myocytes cross-sectional areas(CSA) of indicated groups(n = 8 mice per group, and the number of myocytes is 150 per group). C, Parameters of wall thickness by statistical analysis of LVPWD and IVSD (n = 8). D, Analysis of Anp, Bnp, α-MHC, β-MHC, Serca2α, and Acta1 in WT and KO mice by real-time PCR analysis (n = 6). For B, C and D, Data represent as mean±SEM. *P<0.01 vs. corresponding sham; #P<0.01 vs. WT/AB after AB.
Table 2.
Hemodynamic parameters in mice 28 days post-surgery.
Figure 3.
Increased fibrosis in ELTD1 −/− mice.
A and B, PSR staining and quantitative fibrotic area measurements. Data represent as mean±SEM (n = 8). C RT-PCR analyses of Tgfβ1, Tgfβ2, Colα1, Co3α1, Ctgf, Mmp2, and Mmp9 mRNA levels 28 days after surgeries. GAPDH was used as the sample loading control. (n = 6) *P<0.01 vs. corresponding sham; #P<0.01 vs. WT/AB after AB.
Figure 4.
Effect of ELTD1 on MAPKs and PI3K –Akt signaling pathway.
A, Western blots of MEK1/2, ERK1/2, p38, and JNK phosphorylation and their total protein levels 28 days after surgery in WT and KO mice. GAPDH was used as the sample loading control. (n = 6). Up, representative blots. Down, quantitative results. B, Western blots of Akt,GSK3β, and mTOR phosphorylation and their total protein levels 28 days after surgery. GAPDH was used as the sample loading control (n = 6). Up, representative blots. Down, quantitative results. For A and B, Data represent as the means ± SEM *P<0.01 vs. corresponding sham; #P<0.01 vs. WT/AB after AB.