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Figure 1.

Mean Mouse Grimace Scale scores pre and post vasectomy.

MGS scores are presented on the y-axis (± 1SE) for mice receiving 2 ml/kg Saline, 20 mg/kg Meloxicam and 5 mg/kg Bupivacaine with the pre and post vasectomy recordings on the x-axis (P = 0.002, ★★P = 0.000).

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Figure 1 Expand

Figure 2.

Mean frequency of the composite pain scores pre and post vasectomy.

Composite pain scores are presented on the y-axis (± 1SE) for mice receiving 2 ml/kg Saline, 20 mg/kg Meloxicam and 5 mg/kg Bupivacaine with the pre and post vasectomy recordings on the x-axis (P = 0.001, ★★P = 0.000).

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Figure 2 Expand

Table 1.

The HomeCageScan scored behaviours that significantly decreased in frequency from pre to post vasectomy.

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Table 1 Expand

Table 2.

The HomeCageScan scored behaviours that were unaffected by treatment or time*treatment interaction post vasectomy.

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Figure 3.

Relationship between changes in MGS and manually scored behaviour.

MGS scores of one experienced observer are presented on the x-axis and manually scored behaviours from pre to post vasectomy are presented on the y-axis; composite pain behaviour (a), wound lick (b), grooming (c) and rearing (d).

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Figure 3 Expand

Figure 4.

Relationship between changes in MGS and automatically scored behaviour.

MGS scores of one experienced observer are presented on the x-axis and automatically scored behaviours from pre to post vasectomy are presented on the y-axis; walk left (a), walk right (b), and jump (c).

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Figure 4 Expand

Table 3.

The correlation coefficients and P-values for the automated behaviours that were not significantly correlated with the MGS.

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Table 3 Expand

Table 4.

Ethogram for manual behavioural analysis (adapted from Miller et al. [5]).

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Table 4 Expand

Table 5.

Ethogram for automated behavioural analysis (adapted from Miller et al. [5]).

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