Figure 1.
Priming sites of 23 variable regions in chloroplast genome.
Large white boxes indicate coding areas, small white boxes indicate intergenic spacers, and small black boxes indicate introns. Figures above boxes indicate length (bp).
Table 1.
Angiosperm genera in which complete chloroplast genomes have been determined in two or more species.
Figure 2.
Gel profiles of fragments amplified from six species using 21 pairs of primers.
Numbers shown at top are sequential order of loci as in Table 2 and Fig. 1. Numbers on right are size markers (kbp). Letters on left indicate species as follows: Cp: Chimonanthus praecox (L.) Link; To: Typha orientalis Presl.; Nn: Nelumbo nucifera Gaertn.; Ps: Paeonia suffruticosa Andrews; Pp: Prunus persica (L.) Batsch.; and Pb: Panax bipinnatifidus Seem.
Table 2.
Primers for amplifying and/or sequencing 23 highly variable loci.
Figure 3.
Nucleotide diversity per site (π) and indels and inversions (I) of 21 loci in Nelumbo, Panax, and Prunus.
A & B, Nelumbo, C & D Panax, E & F, Prunus. A, C & E, π; B, D & F I. Four proposed barcoding loci, atpF-atpH, rbcL, rpoB, and rpoC1 were used as controls.
Figure 4.
Maximum parsimony tree of Prunus sect. Persica based on a concatenation of psbM-trnD intergenic spacer and clpP intron, two representatives of the 21 loci.
The figures above the branches are the bootstrap values (NJ|MP) of the clades.