Table 1.
Main features of the five investigated Sardinian soils.
Table 2.
Taxonomic distribution of sequences and OTUs retrieved with the ITS1F-ITS2 (ITS1 region) and ITS3-ITS4 (ITS2 region) primer set in the five Sardinian soils.
Figure 1.
Rarefaction curves describing observed fungal richness.
Rarefaction curves indicating the observed number of operational taxonomic units (OTUs) at a genetic distance of 3% related to the number of sequences retrieved in each of the five different soils with ITS1 (a) and ITS2 (b) primer set, respectively. TV, tilled vineyard; CV, covered vineyard; MM, managed meadow; PA, pasture; CO, cork-oak formation. X-axis = sequences number; Y-axis = OTUs number.
Figure 2.
Taxonomical distribution of retrieved fungi.
Proportion of the (a) 240 ITS1 and (b) 326 ITS2 fungal operational taxonomic units (OTUs) assigned to the different fungal phyla. Ascomycota were dominant (64.5% of total ITS1 OTUs and 68.0% of total ITS2 OTUs), followed by Basidiomycota (30.3% and 25.4%), Zygomycota (4.5% and 6.5%), and Chytridiomycota (0.6% and 0%).
Figure 3.
Dendrogram showing distribution of fungal assemblages.
Classification analysis (UPGMA, chord distance as the resemblance measure) on the basis of OTU relative abundances data. Numbers on branches represent the bootstrap confidence percentage (100 replicates). TV, tilled vineyard; CV, covered vineyard; MM, managed meadow; PA, pasture; CO, cork-oak formation.
Figure 4.
Principal Coordinate Analysis (PCoA) of most abundant fungal OTUs.
Ordination on axes 1 and 2 of soils on the basis of the ITS1 OTU presence (PCoA). High-abundance OTUs (>1% in at least one soil) are considered for the statistical analysis (list of these OTUs is reported in table S1). Low-abundance OTUs (including singletons) are excluded. TV, tilled vineyard; CV, covered vineyard; MM, managed meadow; PA, pasture; CO, cork-oak formation.