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Figure 1.

Distribution of the Otago skink and several closely related species of New Zealand skinks.

The distribution of O. otagense (black circles), O. waimatense (blue squares), O. acrinasum (green circles), O. infrapunctatum (red triangles), O. taumakae (orange square), and O. pikitanga (purple circle) is indicated. Several localities mentioned in the text are highlighted, including the locations of the O. otagense and O. waimatense samples used in this study. The dashed line indicates the estimated former distribution of O. otagense [26]. The distribution data is adapted from the Department of Conservation's BioWeb Herpetofauna database. Inset: Major geographic regions of New Zealand.

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Table 1.

Locality information and GenBank accession numbers for samples used in the phylogenetic analyses.

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Table 2.

Oligonucleotide primers used in this study.

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Figure 2.

Maximum Likelihood (ML) phylogram for the New Zealand skink lineage containing the Otago skink (Oligosoma otagense).

The lineage contains five other species: O. waimatense, O. acrinasum, O. infrapunctatum, O. pikitanga, and O. taumakae. The phylogeny is based upon 1834 bp of mitochondrial DNA sequence data (ND2, ND4 & Cytochrome b). Two measures of branch support are indicated with ML bootstraps (500 replicates) on the left and Bayesian posterior probabilities on the right (only values over 50 and 0.7, respectively, are shown).

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Table 3.

Mean model-corrected genetic distances (ND2, ND4, Cytochrome b) between the Otago skink and several closely-related species.

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Figure 3.

Control region haplotype network for the Otago skink.

Each circle represents one haplotype and the size indicates the number of individuals with each haplotype. The lines indicate one base pair change between sequences. The different colours indicate the population(s) in which each haplotype is found. A) Wild Otago skink populations, B) Wild and captive Otago skinks, C) Wild Otago skinks and introgressed scree skinks.

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Table 4.

Estimates of genetic diversity (mitochondrial control region) within Otago skink populations and regions.

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Table 5.

Mean TrN genetic distances between control region haplotypes.

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Table 6.

Pairwise ΦST among the five Otago skink populations.

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