Figure 1.
Text box listing important aspects of PriMux.
Figure 2.
Diagram of the PriMux approach.
Table 1.
A subset of the most commonly changed user-specifiable parameters in the options file.
Figure 3.
Diagram of the PriMux software workflow.
Table 2.
Rift Valley Fever (RVF) Degenerate Assay Signatures.
Table 3.
Primer and probe (IO) sequences for RVFV run with all segments in a single input file.
Table 4.
Numbers of RVFV targets detected by each primer/probe combination.
Table 5.
Multiplex set of primers and probes to detect all 2863 Dengue virus genomes.
Table 6.
Primer and probe combinations from Table 5, and number and serotypes of Dengue virus genomes detected by each primer/probe combination.
Table 7.
Per Signature Limit of Detection for Rift Valley Fever Strains Tested: All tests were conducted on total nucleic acid extracts from clarified viral cultures containing the listed strains.