Table 1.
Summary of L. longipalpis cDNA library sequencing results.
Table 2.
Results of cluster comparisons to different databases.
Figure 1.
Distribution of the best Blastx matches for assembled Lutzomyia longipalpis ESTs.
The ESTs were submitted to a search against the RefSeq_protein database (NCBI). The e-value cutoff was 1.0e-5.
Figure 2.
Classification of ESTs in Gene Ontology category.
The ESTs of L. longipalpis were submitted to a search against the three categories of Gene Ontology (NCBI). The e-value cutoff was 1.0e-5.
Table 3.
Putative L. longipalpis mRGPs.
Figure 3.
(A) Neighbor-joining tree of putative β-defensins: L. longipalpis 1 (BAR005E03/AM091821, male reproductive organs and whole female cDNA libraries), L. longipalpis 2 (EU124626, midgut female library) and L. longipalpis 3 (EX211140, midgut female library), A. aegypti (AEL009861), A. gambiae (AGAP007049), D. melanogaster (CG10433), and B. mori (NP_001106745). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative β-defensin of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).
Figure 4.
Cyclophilin sequence analysis.
(A) Neighbor-joining tree of putative cyclophilin L. longipalpis (RAAPBAR022E08/AM092289, male reproductive organs and whole female cDNA libraries), A. gambiae (AGAP007088-PA), A. aegypti (AAEL013279), D. melanogaster (FBpp0071844/CG2852) and A. mellifera (NP_001229473). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative cyclophilin of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).
Table 4.
ESTs with other specific function.
Figure 5.
Astacin metalloprotease sequence analysis.
(A) Neighbor-joining tree of putative astacin from L. longipalpis (RAAPBAR022F08 male reproductive organs cDNA libraries), L. longipalpis 2 (AM088883 whole female cDNA libraries) and L. longipalpis 3 (Lulo-Astacin A8CW49_LUTLO, midgut female library) A. aegypti (AAEL013449), A. gambiae (AGAP010764), D. melanogaster (FBpp0080341/CG15254) and Nasonia vitripenis (NV12552). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative astacin of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).
Figure 6.
Protease inhibitor sequence analysis.
(A) Neighbor-joining tree of putative protease inhibitor L. longipalpis (RAAPBAR023H02/EW989852 B male reproductive organs and midgut female cDNA library), A. aegypti (AAEL000551), A. gambiae (AGAP011319), and Apis mellifera (XP_003250953). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative protease inhibitor of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).
Figure 7.
Thioredoxin sequence analysis.
(A) Neighbor-joining tree of putative thioredoxin L. longipalpis (RAAPBAR020D12 male reproductive organs cDNA libraries), A. aegypti (AAEL010777), A. gambiae (AGAP009584-PA) and Tribolium castaneum (XM_962894.2). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative thioredoxin of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).