Table 1.
Lesion size, weight, plasma cholesterol and triglycerides at 14 weeks of age.
Table 2.
Plasma cholesterol, triglycerides and weight at 26 weeks of age.
Figure 1.
CD8+ T cells in spleen and mediastinal lymph nodes (MeLN).
Flow cytometry graphs showing the cell populations in spleen from one representative mouse from each group (A). Numbers given in the graphs are per cent cells out of all lymphocytes. The fraction (B) and total cell count (C) of CD8+ T cells in spleen and MeLN of Apoe−/−, Apoe−/− Tap1−/− and Tap1−/− mice. The cells were isolated from respective tissue, stained with fluorescent antibodies and analyzed by flow cytometry. As expected, the CD8+ T cell fraction was depressed in the mice lacking the Tap1 gene. Each dot in the figure represents one mouse. ***P<0.001.
Figure 2.
Plaque area in aortic root and aorta.
(A) Representative images of plaques in the aortic root from one mouse in each group. Quantification of plaque area in the aortic root (B) and aorta (C) of Apoe−/− and Apoe−/−Tap1−/− mice fed high fat diet for 20 weeks. The tissues were stained with Oil red O (ORO) and analyzed by a blinded observer. Each dot in the figure represents one mouse.
Figure 3.
CD3+ T cells and CD4+ T cells in spleen and MeLN.
Flow cytometry graphs showing the cell populations in spleen from one representative mouse from each group (A). Numbers given in the graphs are per cent cells out of all lymphocytes. The fraction of CD3+ T cells (B), CD3+CD4+ T cells (C) and CD3+CD4+ T cell numbers (D) in Apoe−/− and Apoe−/− Tap1−/− mice in spleen and MeLN. The cells were isolated from respective tissue, stained with fluorescent antibodies and analyzed by flow cytometry. Each dot in the figure represents one mouse. *P<0.05, ***P<0.001.
Figure 4.
CD11c+ cells and expression of CD80+, CD86+ and MHC class II+ on CD11c+ cells in spleen and MeLN.
Histograms corresponding to the cell populations in the spleen from one representative mouse from each group (A). Gate boundaries were set by fluorescence minus one controls (solid grey). The fraction of CD11c+ cells (B) and CD11c+CD80+, CD11c+CD86+ and CD11c+MHCII+ cells in spleen (C) and MeLN (D) of Apoe−/− and Apoe−/− Tap1−/− mice. The cells were isolated from respective tissue, stained with fluorescent antibodies and analyzed by flow cytometry. Each dot in the figure represents one mouse. *P<0.05, **P<0.01.
Figure 5.
Proliferation of splenic CD4+ and CD8+ co-cultures of young mice.
Proliferation were analysed in ConA stimulated (90 hrs) CD4+:CD11c+ (CD4+) and CD8+:CD11c+ (CD8+) cell co-cultures in Apoe−/− and Apoe−/−Tap1−/− mice with presence of radioactive labelled thymidine during the last 16–20 hrs. The cells were isolated from spleens of mice given high fat diet for 8 weeks and magnetically separated into CD11c+, CD4+ and CD8+ cells. CPM denotes counts per minute. Each dot in the figure represents one mouse. *P<0.05.