Figure 1.
Functional cataloging of menadione-responsive genes.
Genes were assigned a putative function based on their homology and classified on the basis of their functions.
Figure 2.
Comparison of gene expression after menadione treatment at different time intervals.
(a) Heat map of hierarchical clustering performed for selected genes illustrates differential induction patterns after menadione-treatment for 0.5 h, 1 h and 3 h. (b) The Venn diagram represents the distribution of transcripts that was significantly induced (>2-fold) at different time intervals.
Figure 3.
Clustering analysis of menadione-responsive gene expression profiles.
(a) SOTA cluster tree of selected genes illustrates differential induction patterns after menadione, H2O2 and NO treatments. Accession numbers provided by NCBI are given in the heat map and the corresponding gene names are listed in Table S1. (b) The 106 genes were grouped into 11 clusters based on their expression profiles. The expression profile of each individual gene in the cluster is depicted by grey lines, while the mean expression profile is marked in pink for each cluster. The number of genes in each cluster is given in the left upper corner and the cluster number in the right lower corner. (c) Functional cataloging of the genes present in different clusters (clusters with n>10 were taken into consideration). (d) The Venn diagram represents the distribution of transcripts that were significantly induced (>2 fold) in macroarray analysis after menadione, H2O2 and NO treatments.
Figure 4.
In planta quantification of expression of genes belonging to cluster 6.
Line diagrams representing the expression pattern of cluster 6 genes from A. rabiei-infected chickpea samples (Pusa-362) are shown as fold-change compared to control. Expression was analyzed at 1, 3 and 6 dpi. Error bars represent ± SE. Ar2, Hypothetical protein SNOG_16463; Ar3, Cytochrome c; Ar11, Cartenoid oxygenase; Ar12, Acetylglutamate kinase; Ar25, Neutral trehalase; Ar26, Hypothetical protein; Ar66, Hypothetical protein ACLA_073190; Ar69, Hypothetical protein SNOG_10250; Ar74, Hypothetical protein; Ar77, C2 domain containing protein; Ar81, Alternative oxidase; Ar96, Plasma membrane ATPase; Ar14, ATP-citrate synthase; Ar48, Hypothetical protein SNOG_00366; Ar65, NADH-ubiquinone oxidoreductase.
Figure 5.
In planta expression analysis of stress responsive genes.
Line diagrams representing the expression pattern of selected genes from A. rabiei-infected chickpea samples (Pusa-362) are shown as fold-change compared to control. Expression was analyzed at 1, 3 and 6 dpi. Error bars represent ±SE. Ar19, FMN dependent dehydrogenase; Ar34, NADH oxidase; Ar35, Catalase; Ar15, Thioredoxin; Ar9, E3 SUMO-protein ligase PIAS1; Ar13, F-box and WD domain containing protein; Ar46, Ubiquitin-conjugating enzyme E2 N; Ar71, Ubiquitin; Ar57, Mannosylphosphate transferase; Ar104, Usp domain-containing protein; Ar36, C6 transcription factor; Ar49, Molecular chaperone BiP; Ar50, 41 kDa peptidyl-prolyl cis-trans isomerase; Ar55, Ribosomal protein S5; Ar86, protein phosphatase PP2A.
Figure 6.
Schematic representation of the putative regulatory and functional networks induced during oxidative stress in Ascochyta rabiei.
This figure summarizes data obtained in the present work and hypothesized mechanisms. Genes are grouped according to their most probable localization and function in the cell. Abbreviation for genes: PP2A, Protein phosphatase PP2A; PKC (C1), Protein kinase C (C1); EsdC, GTP-binding protein EsdC; PKA, RAC-alpha serine/threonine-protein kinase; C6, C6 transcription factor; ZK-TF, Zinc knuckle transcription factor; Rds2, Zn cluster transcription factor Rds2; HSP78, Heat shock protein 78; AOX, Alternative oxidase; MITPC, Mitochondrial phosphate carrier protein; SOD, Superoxide dismutase; CAT, Catalase; ERV14, Endosomal cargo receptor Erv14; BIP, Molecular chaperone BIP; CHS7, Export control protein chitin synthase 7; ACS, ATP-citrate synthase; FAS, Fatty acid synthase; ACD, Acyl-CoA desaturase; COX, Carotenoid oxygenase; ACT, Acetylglutamate kinase; PIAS, E3 SUMO-protein ligase PIAS1; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; Mnn4, Mannosylphosphate transferase; FBO, F-box and WD domain containing protein; TRE, Trehalase; RPS5, Ribosomal protein S5; HSP 70, Heat shock protein 70; SSC1, SSC1-like heat-shock protein; PPI, peptidyl-prolyl cis-trans isomerase; RBOH, Respiratory burst oxidase homolog.