Figure 1.
Chemical structures of PAMAM-G4 dendrimer, cisplatin, curcumin, resveratrol and genistein.
Figure 2.
FTIR spectra and difference spectra (diff.) in the region of 1800-600 cm−1 of hydrated films (pH 7.4) for free mPEG-PAMAM-G3 (A), mPEG-PAMAM-G4 (B) PAMAM-G4 (C) (0.5 mM) and their resveratrol complexes obtained at different polyphenol concentrations (indicated on the figure).
Figure 3.
FTIR spectra and difference spectra (diff.) in the region of 1800-600 cm−1 of hydrated films (pH 7.4) for free mPEG-PAMAM-G3 (A), mPEG-PAMAM-G4 (B) PAMAM-G4 (C) (0.5 mM) and their genisteinl complexes obtained at different polyphenol concentrations (indicated on the figure).
Figure 4.
FTIR spectra and difference spectra (diff.) in the region of 1800-600 cm−1 of hydrated films (pH 7.4) for free mPEG-PAMAM-G3 (A), mPEG-PAMAM-G4 (B) PAMAM-G4 (C) (0.5 mM) and their curcumin complexes obtained at different polyphenol concentrations (indicated on the figure).
Figure 5.
FTIR spectra and difference spectra (diff.) in the region of 1800-600 cm−1 of hydrated films (pH 7.4) for free mPEG-PAMAM-G3 (A), mPEG-PAMAM-G4 (B) PAMAM-G4 (C) (0.5 mM) and their cis-platin complexes obtained at different drug concentrations (indicated on the figure).
Figure 6.
FTIR spectra in the region of 3300-2800 cm−1 of hydrated films (pH 7.4) for free mPEG-PAMAM-G3 (A), mPEG-PAMAM-G4 (B) and PAMAM-G4 (C) and their resveratrol. genistein, curcumin and cisplatin complexes obtained with 0.5 mM polymer and pigment concentrations.
Figure 7.
UV-visible spectra of mPEG-PAMAM-G3, mPEG-PAMAM-G4 and PAMAM-G4 and their complexes with resveratrol and genistein with free dendrimer at 100 mM and complexes b-I at 20 to 130 mM.
(A, B and C) Resveratrol with mPEG-PAMAM-G3, mPEG-PAMAM-G4 and PAMAM-G4 respectively. (D, E and F) Genistein with mPEG-PAMAM-G3, mPEG-PAMAM-G4 and PAMAM-G4 respectively. (A′, B′ and C′) Plots of 1/(A-A0) vs (1/pigment concentration) and binding constant (K) for Res-mPEG-PAMAM-G3, Res-mPEG-PAMA-G4, and Res-PAMAM-G4 respectively. (D′, E′ and F′) Plots of 1/(A-A0) vs (1/pigment concentration) and binding constant (K) for Gen-mPEG-PAMAM-G3, Gen-mPEG-PAMA-G4, and Gen-PAMAM-G4 respectively.
Figure 8.
UV-visible spectra of mPEG-PAMAM-G3, mPEG-PAMAM-G4 and PAMAM-G4 and their complexes with curcumin and cisplatin with free dendrimer at 100 mM and complexes b-I at 20 to 130 mM.
(A, B and C) Curcumin with mPEG-PAMAM-G3, mPEG-PAMAM-G4 and PAMAM-G4 respectively. (D, E and F). Cisplatin with mPEG-PAMAM-G3, mPEG-PAMAM-G4 and PAMAM-G4 respectively. (A′, B′ and C′) Plots of 1/(A-A0) vs (1/pigment concentration) and binding constant (K) for Cur-mPEG-PAMAM-G3, Cur-mPEG-PAMA-G4, and Cur-PAMAM-G4 respectively. (D′, E′ and F′) Plots of 1/(A-A0) vs (1/pigment concentration) and binding constant (K) for cis-mPEG-PAMAM-G3, cis-mPEG-PAMA-G4, and cis-PAMAM-G4 respectively.
Table 1.
Calculated binding constants (K) for drug-dendrimer complexes.
Figure 9.
Optimized polyphenol-PAMAM-G4 docking structures.
The polyphenols are shown in green color. (A) shows whole PAMAM-G4 in spheres with resveratrol and (A′) shows the zoom on the binding site represented in sticks. (B) shows whole PAMAM-G4 in spheres with genistein and (B′) shows the binding site represented in sticks. (C) whole PAMAM-G4 in spheres with curcumin and (C′) shows the binding site in represented in sticks.
Table 2.
Binding energy for the best docking positions for drug-dendrimer Complexes.