Figure 1.
(a) Diagram showing the configuration of water baths and cages for testing cage temperature and nesting material preferences. (b) Diagram depicting elements present in water bath and cage setup. The figures are reproduced with permission from Elsevier [5], [6]
Table 1.
Ethogram of observed behaviors.
Figure 2.
The mean difference in nest score values between the nesting cage and the temperature cage.
Nest scores partitioned by occurrences of nesting material carryover by (a) cage sets and (b) amount of material provided. A negative value indicates a better nest built in the temperature cage and a positive value indicates a better nest in the nesting cage. LSM and SE are plotted and significant t-tests (value different from zero; α corrected for the number of comparisons) are indicated by asterisks. A diagonal line indicates a significant linear trend and a curved line indicates a significant quadratic trend.
Figure 3.
Likelihood of nesting material being transferred to the temperature cage.
Data is plotted by (a) sex and temperature; (b) strain and temperature and; (c) amount of nesting material and strain. Quadratic peaks are indicated by solid vertical lines.
Figure 4.
Location preference due to temperature-set.
Fold difference in percent of location observations between the nesting cage relative to the temperature cage. Effects of temperature-set are plotted by interactions with (a) sex; (b) strain and; (c) behavior. LSM and SE are plotted and significant t-tests (value different from zero-α corrected for the number of comparisons) are indicated by asterisks.
Figure 5.
Location preference by titrated variables.
Mean difference in percent of observations between the nesting cage and the temperature cage for the temperature-set by amount of nesting material interaction. The green area indicates equal preference for NC and TC. Blue and purple shading indicate a 2 and 4 fold preference for NC. Orange and red shading indicate 2 and 4 fold preferences for TC.
Figure 6.
Location preference due to amount of nesting material.
Fold difference in percent of observations between the nesting cage relative to the temperature cage. Effects of the amount of nesting material provided are plotted by interactions with (a) sex; (b) strain and; (c) behavior. LSM and SE are plotted and significant t-tests (value different from zero-α corrected for the number of comparisons) are indicated by asterisks.