Figure 1.
Phylogenetic analysis of JAK family members.
Phylogenetic analysis of JAK protein sequences using the Neighborhood-Joining algorithm, with bootstrap values above 80% (of 1000 replicates) indicated in bold. Species used for phylogenetic analysis are indicated: fruit fly (dm), sea squirt (ci), zebrafish (dr), green pufferfish (tf), Japanese pufferfish (tr), chicken (gg), mouse (mm), and human (hs).
Figure 2.
Synteny analysis of JAK family members.
Synteny analysis of the relevant zebrafish and human JAK gene loci (white), indicating adjacent genes in their respective orientations. Genes showing conserved synteny between zebrafish and human are in black, genes which have homologues displaying synteny with other family members in humans or zebrafish edged in red and non-syntenic genes in grey.
Figure 3.
Phylogenetic analysis of STAT family members.
Phylogenetic analysis of STAT family members as described for Figure 1, with the inclusion of genes from African clawed frog (xl) and Western clawed frog (xt).
Figure 4.
Synteny analysis of STAT family members.
Conserved synteny analysis of STAT loci as described in Figure 2, with the addition of genes that displayed synteny between zebrafish and Japanese pufferfish being shown in green.
Figure 5.
Phylogenetic analysis of SHP family members.
Phylogenetic analysis of SHP family members as described for Figure 3.
Figure 6.
Synteny analysis of SHP family members.
Syntenic analysis of SHP family members as described for Figure 4.
Figure 7.
Phylogenetic analysis of PIAS family members.
Phylogenetic analysis of PIAS family members as described for Figure 3.
Figure 8.
Synteny analysis of PIAS family members.
Syntenic analysis of PIAS family members as described for Figure 4.
Figure 9.
Phylogenetic analysis of SOCS family members.
Phylogenetic analysis was performed on SOCS family members as described for Figure 3.
Figure 10.
Synteny analysis of SOCS family members.
Syntenic analysis of SOCS family members as described for Figure 4.
Figure 11.
A model for the evolution of the of JAK, SHP, and PIAS families.
(A) Evolutionary timeline summarizing key events in chordate/vertebrate evolution. Key evolutionary events are shown, including the divergence of protostomes (solid grey line) from deuterostomes, the subsequent divergence of urochordates (broken grey line) from other chordates, and finally ray-finned fish (including teleosts) (dotted black line) from lobe-finned fish (including terapods) (black line), with relevant present-day groupings (insect, urochordate, teleost, and tetrapod) indicated. Whole genome duplications (1R, 2R, 3R) are indicated with short red lines. (B–D) Model for the evolution of JAK-STAT pathway components: JAK (B), SHP (C), and PIAS (D) families. Red ovals indicate the likely core members at the time of protostomes/deuterostomes and urochordate/chordate divergence, while the grey shaded rectangle represents ray-finned/lobe-finned fish ‘core’ signaling components present at the time of divergence. Lineage-specific/local duplications are indicated by red dots and gene conversion indicated by a red square.
Figure 12.
A model for the evolution of the STAT family.
A model for the evolution of the STAT component of the JAK-STAT pathway as described in Figure 11.
Figure 13.
A model for the evolution of the SOCS family.
A model for the evolution of the SOCS component of the JAK-STAT pathway as described in Figure 11.