Figure 1.
Gating strategies for major lymphocyte populations.
Gating strategies for major lymphocyte populations in peripheral blood and lymph node respectively. Values are given as percent of lymphocytes. For CD56bright subpopulation value is presented as percent of CD56. Examples from one ACS (acute coronary syndrome) patient shows dot plots representative for both SA (stable angina) and ACS patients.
Figure 2.
Gating strategies for regulatory T cells.
Different strategies for gating regulatory T cells in peripheral blood and lymph node respectively. Panel A; Regulatory T cells is based on CD3 expression (not shown) ,slightly lower (dim) CD4 expression and high (bright) expression of CD25, termed CD4dim/CD25bright [18] Panel B; Regulatory T cells based on CD3 expression (not shown), Foxp3 expression on CD4 population and with limit of the CD8 population ( = old gating strategy). Panel C; Regulatory T cells is based on CD3 expression (not shown), high (bright) expression of CD25 (not shown), slightly lower (dim) CD4 expression and high Foxp3 expression. Values are given as percent CD3+4+. Examples from one ACS (acute coronary syndrome) patient shows dot plots representative for both SA (stable angina) and ACS patients.
Table 1.
Characteristics of SA and ACS patients.
Table 2.
Distribution of major lymphocyte subpopulations.
Table 3.
Distribution of T cell activation markers and regulatory T cells.
Figure 3.
Gating strategy for Foxp3 and CD69.
Gating strategy for Foxp3 and CD69 distribution on regulatory T cells in peripheral blood and lymph node respectively. The CD69/Foxp3 subset is based on CD4dim/CD25bright (Fig. 2A). Values are given as percent CD4dim/CD25 bright. Examples from one ACS (acute coronary syndrome) patient shows dot plots representative for both SA (stable angina) and ACS patients.
Table 4.
Distribution of type 1 lymphocyte subsets.