Table 1.
Concentrations of the eight calibrators (ng/mL) prepared with defibrinated and charcoal stripped human serum.
Table 2.
Multiple-reaction monitoring (MRM) transitions, declustering potentials (DP), collision energies (CE) and collision cell exit potential (CXP) for the 8 steroids and deuterated internal standards.
Table 3.
Correlation coefficients (R) obtained from an eight-point calibration curve, recovery (in percent), intra-assay and inter-assay precision (coefficient of variation; CV) for each steroid.
Table 4.
Serum and plasma samples were processed by liquid-liquid extraction (using ethyl acetate or diethyl ether), or by solid phase extraction (SPE) using water as the washing solvent, or by SPE using water and hexane as washing solvents (sequentially).
Figure 1.
Ion chromatograms for a reindeer serum pool that included two males and two non-reproductive females.
Cortisol, corticosterone, 11-deoxycortisol, progesterone, 17-OH progesterone and testosterone were present in this sample, but estradiol and DHEA were either not present or below the limit of quantitation. Transition details are presented in Table 2 and retention times (minutes) are indicated above the peak.
Table 5.
Steroid levels in mammalian sera or avian plasma (ng/mL).