Figure 1.
Time to loss of righting reflex (LORR) in seconds within the 3 treatment groups and the 2 strains of mice (C57Bl/6 and CD1).
Error bars: +1 SD. Composition of the gas mixtures for the groups 1–3: see table 3.
Table 1.
Values of pH, PaO2 (mmHg) and lactate (mmol.l−1) measured in arterial blood at the time of loss of righting reflex.
Figure 2.
Diagram representing the induction chamber.
The fresh gas inlet (bottom arrow) of the chosen mixture was delivered underneath a multi-perforated floor. A total of 4 mini fans were ensuring that the mixture was optimally blended. The sampling end of the sidestream gas monitoring (X) was positioned at mouse level, 8 cm above the floor. The gas outlet (upper arrow) was connected to an active scavenging system.
Table 2.
Composition and flow rate of the anaesthetic mixtures used for anaesthesia induction – pilot study.
Table 3.
Composition and flow rates of the gas mixtures used for mice euthanasia- main study.