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Table 1.

Observational battery average results of all mice treated with scopolamine or vehicle.

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Figure 1.

Results in the Rota rod.

Significant differences in rota rod performance, in scopolamine-treated vs vehicle-treated groups (P≤0.05) are shown. Numbers are representing seconds of remaining on the revolving rod.

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Figure 2.

Results in the MTM.

Significantly higher latencies and longer pathlengths were observed in the scopolamine-treated animals (A,B). Correct decisions to reach the goal box were higher in vehicle-treated and trained mice (C) and the number of successful entries into the goal box during training days was significantly higher in the vehicle-treated and trained mice (D). At the probe trial latencies were significantly lower in the vehicle-treated and trained mice (E).

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Figure 3.

Western blot results of M1.

The M1 receptor complex levels were significantly higher in scopolamine-treated groups.

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Figure 4.

Western blot results of Nic 7.

Nic7 complex levels were significantly higher in scopolamine-treated groups. Significant differences were also observed when scopolamine-treated trained and untrained mice were compared.

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Figure 5.

Western blot results of Nic 4.

Although a trend was suggested no significant differences between groups were observed.

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Figure 6.

Western blot results of NR1.

NR1 containing NMDA complex levels (band 1) were significantly increased in the scopolamine-treated trained group vs the vehicle-treated and trained panel (A). Band 2 (B) was not significantly different between groups.

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Figure 7.

Loading control.

The membrane used for immunoblotting was stained by Coomassie blue R-350. Adaequate loading was shown.

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Figure 8.

Significant correlations.

(A) The significant correlation between Nic4 and M1 complex levels as well as (B) the significant correlation between NR1 (band 2) and Nic7 is demonstrated.

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Figure 9.

Scheme of multiple T-maze.

The multiple T-maze is a landmaze paradigm testing spatial memory. Animals with food deprivation search for food that is provided in the goal box.

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