Figure 1.
Diagrammatic representation of fungal phylogeny based on ref. 18 and of their putative globins.
Estimates of the numbers of species in parentheses are from the Dictionary of the Fungi [23]. Red bar indicates multicellularity with differentiated tissues; FHb* - unknown N-terminal domain linked to a FHb with an incomplete reductase domain, T1gb - T globin group1. Ratios refer to the number of genomes containing globins versus the total number of genomes analyzed.
Figure 2.
Diagrammatic representation of FHb structures.
Schematically displayed structures are those of a normal FHb and of an abnormal chimeric FHb missing the C-terminal moiety of the reductase domain found in 21 of 29 Saccharomycotina genomes. The normal FHb is represented by E. coli FHb (PDB: 1gvh).
Figure 3.
Bayesian phylogenetic tree of fungal FHbs.
Bayesian tree based on a MAFFT v.6.850 alignment of 62 fungal FHb globin domains using two plant nonsymbiotic Hbs as outgroup. Support values at branches represent Bayesian posterior probabilities (>0.5). The sequences are identified by the first three letters of the binary species name, the number of residues, and the full phylum and family names (see Table S1). Sac – Saccharomycetes.
Figure 4.
Bayesian phylogenetic tree of fungal and bacterial FHbs.
Bayesian tree based on a T-COFFEE 9.01 alignment of the globin domains of 55 representative fungal FHbs and 54 representative bacterial FHbs. Support values at branches represent Bayesian posterior probabilities (>0.5). All the bacterial FHbs are in the blue boxes. The sequences are identified by the first three letters of the binary species name, the number of residues, and the full phylum and family names (see Table S1). Sac – Saccharomycetes.
Figure 5.
Bayesian phylogenetic tree of fungal Sgbs.
Bayesian tree based on a MAFFT v.6.850 alignment of 59 fungal, one rotifer and one heterolobosan Sgbs, using two bacterial Pgbs as outgroup. Support values at branches represent Bayesian posterior probabilities (>0.5). The sequences are identified by the first three letters of the binary species name, and the full phylum and family names (see Table S1).
Figure 6.
Bayesian phylogenetic tree of fungal and bacterial Sgbs.
Bayesian tree based on a T-COFFEE 9.01 alignment of 51 fungal, 57 bacterial (including 16 Pgbs), one rotifer and one heterolobosan Sgbs, using two Adgb sequences [16] as outgroup. Support values at branches represent Bayesian posterior probabilities (>0.5). The sequences are identified by the first three letters of the binary species name, the number of residues, and the full phylum and family names (see Table S1).
Figure 7.
Bayesian phylogenetic tree of fungal and bacterial T1 globins.
Bayesian tree based on a T-COFFEE 9.01 alignment of 2 fungal (red and black arrows), 70 bacterial (blue), 4 euryarchaeote (purple) and 10 chlorophyte (green) T1 globins, using 2 Physcomitrella nsHbs as outgroup. Support values at branches represent Bayesian posterior probabilities (>0.5). All the bacterial FHbs are in the blue boxes. The sequences are identified by the first three letters of the binary species name, the number of residues, and the full phylum and family names (see Table S1).
Table 1.
Bacterial phyla whose globins may share ancestry with fungal globins.