Table 1.
Genes down regulated in S. coelicolor M28 and up regulated in S. coelicolor I32.
Figure 1.
Antibiotic production in the deficient strain.
(A) Growing cultures and actinorhodin production of S. coelicolor I32 (black circles) or its isogenic S. coelicolor M145 (white circles) in R5 liquid medium at 30°C. (B) Growing cultures and undecylprodigiosin production of S. coelicolor I32 (black circles) or its isogenic S. coelicolor M145 (white circles) in R5 liquid medium at 30°C. The data presented are the average of at least three independent determinations; the bars show standard error.
Figure 2.
Antibiotic production in the SCO5785 overproducer strain.
(A) Growing cultures and actinorhodin production of S. coelicolor M28 (black circles) or its isogenic S. coelicolor M145 [pIJ487] (white circles) in R5 liquid medium at 30°C. (B) Growing cultures and undecylprodigiosin production of S. coelicolor M28 (black circles) or its isogenic S. coelicolor M145 [pIJ487] (white circles) in R5 liquid medium at 30°C. The data presented are the average of at least three independent determinations; the bars show standard error.
Figure 3.
Sporulation of the SCO5785 deficient and overproducer strains.
(A) The strain carrying the regulator in multicopy (S. coelicolor M28) showed a premature sporulation phenotype when compared to the isogenic strain, while the deficient strain (S. coelicolor I32) appeared to have delayed sporulation when compared to that of the wild type strain (B). Plates were incubated in MS medium at 30°C at the indicated times.
Table 2.
Genes encoding secretory proteins modulated by SCO5785.
Table 3.
Leader peptides of the secretory proteins modulated by SCO5785.