Table 1.
Plasma parameters in CCL3−/−→LDLR−/− mice.
Figure 1.
Recipients of CCL3−/− bone marrow have reduced lesion area.
LDLR−/− mice were transplanted with C57BL/6 or CCL3−/− bone marrow according to the Methods section. At 4 weeks post-BMT, mice were placed on WD for 6 or 12 weeks. Aortic root lesion area was quantified according to the Methods section. Panels A and B show lesion area from female and male mice after 6 weeks of WD feeding, respectively. Data are presented as mean ± SEM from 7–14 mice per group. Panels C and D show the lesion area from female and male mice after 12 weeks of WD feeding, respectively. Data are mean ± SEM from 7–13 mice per group.
Figure 2.
Lesion complexity does not differ with CCL3 bone marrow genotype.
LDLR−/− mice were transplanted with C57BL/6 or CCL3−/− bone marrow. At four weeks post-BMT, mice were placed on WD for 12 weeks. ORO (4×), Trichrome, MOMA-2, and CD4 stained lesion (10×) are in columns as indicated on figure. Gender and donor marrow are indicated on the left of the figure. Sections were chosen from images representing mice with lesion areas close to the mean of their respective groups.
Figure 3.
Recipients of CCL3−/− marrow have reduced lesional T lymphocytes.
Sections from the aortic root of male and female CCL3+/+→LDLR−/− and CCL3−/−→LDLR−/− mice were used for immunofluorescent analysis of CD4 positive T-cells according to the Methods section. For each mouse, data from the three valves was combined. Total dapi stained nuclei (Panel A) and CD4 positive cells were quantified (Panel B). The percent of cells that were CD4 T cells was calculated by dividing the total cells by the CD4 positive cells (Panel C). Data represent the mean ± SEM of 3–6 mice per group. * P<0.01 compared to male CCL3+/+→LDLR−/−.
Figure 4.
Adipose tissue mass and gene expression.
Male LDLR−/− mice were transplanted with C57BL/6 or CCL3−/− bone marrow. At 4 weeks post-BMT, mice were placed on WD for either 6 or 12 weeks. (A–C) Body weight, total body lean mass, and total body adipose tissue were measured by NMR pre-BMT, 4 weeks post-BMT, and at 6 and 12 weeks post-WD. Data from pre-BMT, 4 weeks post-BMT, and 6 weeks post-WD are combined for the 6 week and 12 week groups. The 12 week post-WD data represent only the mice sacrificed at that time point. (D–F) Perigonadal adipose tissue was collected from the mice fed WD for 12 weeks. RNA was isolated and used for real-time RT-PCR analysis as described in the Methods section. Data are the mean ± SEM of the relative gene expression for 12–14 mice per group. Images are from Toluidine Blue O stained sections processed as described in the Methods section. * P<0.05 ** P<0.01 *** P<0.0005 CCL2 = monocyte chemoattractant protein-1 TNF-α = tumor necrosis factor-α Man Rec C = mannose receptor C Mgl = macrophage galactose N-acetyl-galactosamine specific lectin.
Table 2.
Body and tissue weights in CCL3−/−→LDLR−/− mice.
Figure 5.
Lipid accumulation and gene expression in liver.
Liver was collected from the male mice fed WD for 6 or 12 weeks. (A–B) Liver TG was quantified by GC-MS as described in the Methods section. (C) RNA was isolated and used for real-time RT-PCR analysis as described in the Methods section. Data are the mean ± SEM of the relative gene expression for 8–12 mice per group. CCL2 = monocyte chemoattractant protein-1 TNF-α = tumor necrosis factor-α SREBP-1c = sterol regulatory element binding protein-1c * P<0.05 ** P<0.01 *** P<0.001.
Figure 6.
Stem cell progenitors in CCL3−/−→LDLR−/− mice.
Male LDLR−/− mice were transplanted with C57BL/6 or CCL3−/− bone marrow. At 6 weeks post-WD, bone marrow cells were collected and analyzed by flow cytometry. (A) Quantification of flow cytometry analysis of HSC cells which were identified as lineage negative (Lin−) and expressing high levels of c-kit and Sca-1 (Lin−c-kithighSca-1+). (B) CMP cells were identified as Lin−c-kithighCD34+FcγRII/IIIlo. (C) MEP cells were defined as Lin−c-kithighCD34−FcγRII/IIIlo. (D) GMP cells were defined as Lin−c-kithighCD34+FcγRII/IIIhigh. Data represent the mean± SEM of 5–6 mice per group. *P<0.05 compared to C57BL/6−/− groups.
Table 3.
Plasma parameters in C67BL/6→LDLR−/−, CCL3−/−→LDLR−/−, and CCL2−/−→LDLR−/− mice at 6 weeks post WD-feeding.