Figure 1.
NINA heating devices with DaqPRO 5300 Data recorder.
Table 1.
Sequence of HIV-1 reverse transcriptase-specific RT-LAMP primers.
Table 2.
Effect of ambient temperature on amplification efficiency and reproducibility in NINA heaters.
Figure 2.
Representative temperature profile of RT-LAMP reaction in NINA heater.
The temperature of the reaction tube and phase change material (PCM) was recorded for a 60 minute RT-LAMP reaction using the DaqPRO 5300. Temperature recording was initiated following addition of water to the CaO and terminated upon sample removal.
Table 3.
Effect of ambient temperature on stability and reproducibility of reaction temperature in NINA heaters.
Figure 3.
Validation of NINA heaters with clinical specimens.
The amplification results from a representative clinical specimen are displayed in the figure. Whole blood from an HIV-1 infected patient was added directly into the RT-LAMP reaction and amplification in all 3 NINA heaters was compared. Amplified material was analyzed by (A) agarose gel electrophoresis or (B) by observing fluorescence in the reaction tubes under a UV light.
Table 4.
Amplification of HIV-1 DNA/RNA from whole blood in NINA heaters.