Figure 1.
Illustrating the segmentation of the murine liver lobes according to the Nomina Anatomica Veterinaria.
A Since in most animals we have not been able to identify the quadrate lobe in micro-CT imaging or in situ, the quadrate lobe was put in brackets and not color-coded. B and C are photographs of the liver of a C57BL/6J mouse (ventral view). In C the left and the right liver lobe have been folded cranially to reveal the liver lobes lying below. The single liver lobes have been consistently color-coded according to the schematic segmentation (1A) and to the related micro-CT figures 2, 3, 4 to simplify orientation.
Figure 2.
Coronally reconstructed micro-CT images.
Imaging of the liver of a C57BL/6J mouse was performed 3 hours after i.v. injection of 100 µl of a nanoparticular contrast agent (ExiTron nano 12000). The color-coding of the single liver lobes corresponds to the color coding presented in Figure 1. The slice orientation in relation to the spine is shown in the lower right corner.
Figure 3.
Saggital reconstructed micro-CT images.
Micro-CT of the liver of a C57BL/6J mouse was performed 3 hours after injection of 100 µl of a nanoparticular contrast agent (ExiTron nano 12000). The color-coding of the single liver lobes corrsponds to the color coding presented in Figure 1. The slice orientation in relation to a coronal section is shown in the lower right corner.
Figure 4.
Axial reconstructed micro-CT images.
Micro-CT of the liver of a C57BL/6J mouse was performed 3 hours after injection of a nanoparticular contrast agent (ExiTron nano). The color-coding of the single liver lobes corrsponds to the color coding presented in Figure 1. The orientation of the axial slices in relation to the spine is shown in the lower right corner.
Figure 5.
A–C are comparable coronal sections of the murine liver illustrating the variability of the size of the papillary process of the caudate lobe. D shows transdiaphragmatic herniation of parts of the right medial liver lobe, which we incidentally discovered during our studies.
Figure 6.
A shows coronally reconstructed micro-CT images acquired 20 minutes after injection of 400 µl Fenestra VC. The portal vein (arrowheads) runs in a caudocranial direction and divides into single veins supplying the liver lobes with blood from the abdominal organs. The right branch (1; Ramus dexter) divides up into two non-specified branches supplying the right lateral lobe (colored green) and the right caudate process (colored cyan). The left branch (2; Ramus sinister) divides into the umbillical part (3; Pars umbillicalis) and the transversal part (4; Pars transversalis) draining the left medial lobe and the left lateral lobe, respectively. The vein supplying the right medial liver lobe (colored blue) is not classified in the NAV. Spontaneous peristaltic movement of the portal vein is known to result in a corkscrew-like appearance of the vessel as shown in B. The liver is supplied with arterial blood from the coeliac artery (arrow) arising from the aorta cranial of the cranial mesenteric artery (arrowhead), as shown in C. The portal vein collecting blood from smaller abdominal vessels can be spotted directly ventral to the hepatic artery. D shows a coronally reconstructed micro-CT, 21 days after injection of 100 µl Exitron nano 12000 showing relevant organs adjacent to the liver. The right kidney (1), the left kidney (2), the spleen (3), the stomach (4; containing air bubbles), and the adrenal glands (arrow heads). The dotted lines indicate the renal impression of the liver on the right side and the gastric impression on the left side.
Figure 7.
Maximum intensity projection (MIP) of micro-CT images of the murine hepatic veins 20 minutes after injection of 400 µl Fenestra VC. The color coding of the draining veins corresponds to the color coding used for the liver lobes before. The renal veins are indicated by arrowheads.
Figure 8.
Illustrative micro-CT images of liver metatstases in a mouse.
A–C Images of liver metastases were acquired 2 hours after i.v. injection of 100 µl of a contrast agent (Viscover Exitron nano 6000; Miltenyi Biotec, Bergisch-Gladbach, Germany) in a coronal (A), transversal (B), and saggital (C) slice orientation. With regard to liver anatomy, we found metastases to develop predominantly under the liver capsule adjacent to the fissures between the liver lobes. While the left lateral lobe (LLL) shows no metastases on its caudal edge in this slice, there are plenty on its cranial edge, next to the left medial lobe (LML). The lobes on the right side (right medial lobe (RML), right lateral lobe (RLL) and the caudate process (CP)) show (besides the subcapsular growth pattern) metastases within the liver parenchyma. Due to tumor growth the papillary process depicted in B and C lost its typical shape (compare with figs. 2, 3, 4).