Figure 1.
Schematic of the developed bioreactor.
A. Bioreactor including T-flask, substrate, voice-coil actuator, linear stepper motors, rotary stepper motors, and scissor bars. B. Experimental setup, with static attachment in place of stepper motors. Non-vibrated controls can also be seen. C. Bioreactor within incubator. Wave-form generator is next to the incubator, sitting on top of the power amplifier.
Table 1.
Primer sequences and product sizes for RT-PCR.
Figure 2.
Proliferation of hVFF and BM-MSC.
A. Representative 20× of vibrated and control immunohistochemistry for each cell type. Proliferating cells are marked by TRITC (red), cell nucleus (blue DAPI). B. Percentage of proliferating cells under each condition. An increase in proliferation is observed with vibrated hVFF compared to controls. *(p = .011).
Figure 3.
A. Representative 20× pictures of vibrated and control immunohistochemistry sections for each cell type. Apoptotic cells are marked by FITC (green), cell nuclei DAPI (blue). B. The percentage of proliferating cells under each condition. An increase in apoptosis is observed with vibrated BM-MSC compared to controls. * (p = .0402).
Figure 4.
Gene Expression for hVFF and BM-MSC.
A. Box and whisker plot showing expression changes for CIα1. No statistical difference was found between vibrated and control for either cell type. B. Box and whisker plot showing expression changes for FN. No statistical difference was measured between vibrated and control for either cell type. C. Box and whisker plot showing expression changes for TGF-β1. No statistical difference was measured between vibrated and control for either cell type.