Figure 1.
A construct containing a URA3 gene flanked by identical AluSp repetitive elements was inserted into the low-copy-number yeast plasmid pRS415.
Table 1.
Alu-mediated recombination rates and genetic analyses of candidate suppressors of Alu mediated recombination.
Table 2.
PIF1 variants.
Figure 2.
pfh1-L430P does not complement wild-type pfh1+ function.
(A) Complementation assay in pfh1::loxP pfh1+ kanMX6 loxP strain with either the L430P mutation (leu::pfh1-L430P), Pfh1 (leu::pfh1+), or an empty vector (leu::leu+) strain after transformation with a plasmid expressing Cre (cre+) or Cre-Y324F (cre−). The transformation plates are contrast-inverted pictures. (B) Anti-Pfh1 western blot of wild-type Pfh1GFP (WT) and three independent clones (1–3) expressing Pfh1-L430P. S. pombe cells were grown in the absence (−) or presence (+) of thiamine. 0, 12, and 24 hours indicates the amount of time cells were grown in the presence of thiamine. The loading control (LC) is a non-specific background band. (C) Heterologous expression constructs of pfh1-nuc or pfh1-mt* are indicated in the presence of pfh1::loxP pfh1+ kanMX6 loxP and leu::pfh1-L430P. Contrast-inverted pictures of transformation plates with cre+ on the top and cre− on the bottom.