Figure 1.
Absorbance and fluorescence emission spectra of CdTe-GSH QDs synthesized at 90°C.
A, samples were withdrawn at the indicated times. B, fluorescence of the as-produced CdTe-GSH QDs at the indicated times of synthesis and excited with UV light at 312 nm. C, Dynamic Light Scattering (DLS) analysis of green, yellow and red CdTe-GSH nanoparticles.
Table 1.
EDS elemental analysis of the as-prepared CdTe-GSH NPs. The element per cent in green, yellow and red CdTe-GSH NPs was determined.
Figure 2.
IR spectra of CdTe-GSH NPs and GSH.
Figure 3.
Absorbance and fluorescence emission spectra of CdTe-GSH QDs synthesized at different pHs at 90°C.
A, above, CdTe-GSH QDs after 2 h; below, same CdTe-GSH QDs after 4 h. Samples prepared at pH 13 showed no fluorescence at 4 h. B, Effect of nucleation temperature on CdTe-GSH absorbance peaks measured at different times of synthesis.
Figure 4.
Uptake and intracellular localization of CdTe-GSH QDs by MKN45 cells.
A, Confocal fluorescence images of MKN45 cells incubated with 100 µg/ml CdTe-GSH QDs in the absence (100 QD) or presence of lipofectamine (100 QD+L). QDs are shown in green and cell cytoplasm was stained by phalloidin (red). B, MKN45 cells incubated with 100 µg/ml CdTe-GSH QDs plus lipofectamine (100 QD+L). QDs are shown in green and cell nuclei were stained by PI (red).
Figure 5.
Characterization of QDs incorporation into MKN45 cells by flow cytometry.
A, Per cent of cells incorporating QDs. B, MFI represent the amount of QDs incorporated after incubation with 25, 50 and 100 µg/ml QDs in the presence of lipofectamine. C, Viability of MKN45 cells incubated with QDs with or without lipofectamine. The total population of cells (QD+PI−, QD+PI+ and QD−PI+) is referred to as 100%. Numbers indicate concentrations (µg/ml) and L stands for lipofectamine. D, Characterization of cell death in MKN45 cells incubated with QDs plus lipofectamine. 100% stands for the total population of QD+ cells.