Figure 1.
Liver regrowth following partial hepatectomy (PH).
Mice were subjected to PH and sacrificed at the indicated time points. Liver and body weights were recorded. Liver-to-body weight ratio was used as a liver regrowth index. The results are presented as mean liver-to-body weight ratios ± SD (n = 3 mice per time point).
Figure 2.
Hepatocyte proliferation induced by partial hepatectomy (PH).
Mice were subjected to PH and sacrificed at the indicated time points. Ki-67 immunostaining was performed with formalin-fixed and paraffin-embedded liver sections. Ki67–positive hepatocytes were counted at 200× magnification in 5 randomly chosen fields per section. The results are shown as means per field ± SD (n = 3 mice per time point).
Figure 3.
Dynamic of hepatocyte mitosis during partial hepatectomy (PH)-induced liver regeneration.
Mice were subjected to PH and sacrificed at the indicated time points. Hepatic mitotic figures were counted at 100× magnification in 5 randomly chosen fields per liver section stained with hematoxylin and eosin. The data are shown as means per field ± SD (n = 3 mice per time point).
Figure 4.
Hepatic expression of cell cycle components after partial hepatectomy (PH).
Livers were collected at the indicated time points from normal mice and the mice subjected to PH. Liver lysates with equal amounts of protein from three mice per time point were pooled. Western blotting was performed with antibodies against the proteins indicated. Glyceraldehyde 3-phosphate dehydrogenase (GADPH) was used as a loading control. NL, normal liver.
Figure 5.
Protein expression of Bmal1, Wee1, Cdc2, and Cdc2 phosphorylated at Tyr 15 (p-Cdc2) in regenerating livers.
Livers were collected at the indicated time points from normal mice and the mice subjected to PH. Liver lysates with equal amounts of protein from three mice per time point were pooled. Western blotting was performed with antibodies against the proteins indicated. Glyceraldehyde 3-phosphate dehydrogenase (GADPH) was used as a loading control. NL, normal liver.
Figure 6.
Distribution of Bmal1 protein in regenerating livers and hepatocytes.
Bmal1 immunostaining was performed on liver sections prepared from formalin-fixed and paraffin-embedded hepatic tissues collected from mice sacrificed at the indicated time points after partial hepatectomy. 100× and 400× represent original magnifications.
Figure 7.
Histological analysis of regenerating livers.
Livers were isolated at the indicated time points from mice that underwent partial hepatectomy. Formalin-fixed and paraffin-embedded liver sections were prepared and subjected to hematoxylin and eosin staining (400× original magnification).
Figure 8.
Hepatic fat accumulation in regenerating livers.
Livers were isolated at the indicated time points from mice that underwent partial hepatectomy. Frozen liver sections were prepared and used for Oil red O staining (200× original magnification).