Figure 1.
Bone marrow tissue contributions to tumor stromal elements.
Lethally irradiated RFP+ mice were reconstituted with GFP+ bone marrow (n = 3). After engraftment, ID8 cells were injected subcutaneously. 5 weeks later, tumors were harvested, and sections were analyzed for α-SMA, NG2, FAP, FSP, CD31, and F4/80 co-staining with GFP+ bone marrow derived cells or RFP+ non bone marrow derived cells. (A&B) Red arrows represent co-staining of GFP+ bone marrow derived cells with FAP, FSP or F4/80. Representative images are shown for 2 separate animals.
Figure 2.
Non-bone marrow tissue contributions to tumor stromal elements.
Lethally irradiated RFP+ mice were reconstituted with GFP+ bone marrow (n = 3). After engraftment, ID8 cells were injected subcutaneously. 5 weeks later, tumors were harvested, and sections were analyzed for α-SMA, NG2, FAP, FSP, CD31, and F4/80 co-staining with GFP+ bone marrow derived cells or RFP+ non bone marrow derived cells. (A&B) Yellow arrows represent co-staining of RFP+ non-bone marrow derived cells with α-SMA, NG2 or CD31. Representative images are shown for 2 separate animals.
Table 1.
Quantitative analysis of the origin of stromal elements.
Figure 3.
BM MSC contribution to the tumor microenvironment.
Prospectively isolated and in vitro isolated MSC (RFP+) were combined with whole marrow and transplanted into lethally irradiated mice (n = 3 for each of the 2 groups). After engraftment, ID8 cells were subcutaneously injected. After 5 weeks of tumor groups, sections from harvested tumors were analyzed for RFP+ cells. (A) Representative images are shown for RFP co-staining with FAP and FSP as indicated by the white arrows in the merged images. There is no co-staining between RFP+ cells and α-SMA. (B) Scores were assessed for the relative percentage of RFP+ cells co-staining with each stromal marker.
Figure 4.
Engrafted GFP+ adipose tissue is locally recruited into the tumor microenvironment.
GFP+ adipose tissue was subcutaneously implanted into wild type mice (n = 3). After engraftment, E0771 cells were subcutaneously injected. Two weeks later, sections of resected tumors and adjacent adipose tissue were analyzed for GFP expression. Analysis revealed engrafted adipose tissue with normal morphology. Recruited GFP+ adipose derived cells remained in close proximity to the transplanted adipose tissue. White arrows in the montage image indicate adipose-derived tumor stroma participants.
Figure 5.
Stromal marker expression of recruited GFP+ adipose derived cells.
GFP+ adipose-derived cells displayed (A & B) a high degree of overlap in expression of CD31, α-SMA, and NG2 as indicated by the white arrows in the merged column and (C & D) minimal overlap with FAP, FSP, and F4/80. Representative images are shown for 2 separate animals.
Table 2.
Recruited adipose derived cell expression of stromal markers.
Figure 6.
A tumor is composed of not only cancer cells, but also recruited host-derived cells. Our model suggests that the majority of pericytes (NG2+ and α-SMA+) and endothelial cells (CD31+) are recruited from local tissue, such as local adipose tissue. FAP+ and FSP+ fibroblastic cells involved in extracellular matrix remodeling are recruited from host bone marrow populations, such as BM MSC.